索拉非尼抑制人非小细胞肺癌细胞的增殖并诱导其凋亡  

Sorafenib inhibits the growth of human non-small cell lung cancer cells and induces their apoptosis

作　　者：祁慧薇[1] 范理宏[2] 

机构地区：[1]上海交通大学附属第六人民医院肺内科,上海200233 [2]上海市肺科医院肿瘤科,上海200433

出　　处：《肿瘤》2011年第12期1077-1081,共5页Tumor

摘　　要：目的:观察索拉非尼对人非小细胞肺癌(non-small cell lung cancer,NSCLC)A549和H1299细胞增殖和凋亡的影响,并探讨其可能机制。方法:不同浓度索拉非尼作用A549和H1299细胞后,应用CCK-8(cellcountingkit-8)法检测细胞的增殖抑制率,FCM检测细胞周期和细胞凋亡,蛋白质印迹法检测细胞的磷酸化细胞外信号调节激酶(phosphorylated extracellular signal-regulated kinase,p-ERK)和磷酸化Ak(tphosphorylated Akt,p-Akt)蛋白的表达水平。结果:不同浓度索拉非尼能抑制A549和H1299细胞的增殖,且呈浓度依赖性(P<0.05);索拉非尼能诱导细胞凋亡,与对照组比较,G0/G1期细胞比率明显上升,S期细胞比率相应下降,细胞阻滞于G0/G1期(P<0.05);索拉非尼作用后,A549和H1299细胞中p-ERK蛋白的表达明显低于对照组(P<0.05)。结论:索拉非尼能抑制人NSCLCA549和H1299细胞的增殖并诱导其凋亡,其作用机制可能与阻断ERK信号通路有关。Objective： To observe the effects of sorafenib on the proliferation and apoptosis of human non-small cell lung cancer （NSCLC） A549 and H1299 cells and to explore their possible mechanisms. Methods： The A549 and H1299 cells were treated with sorafenib at different concentrations. The inhibitory rate of cell proliferation was assessed by cell counting kit-8 （CCK-8）. The apoptosis and the cell cycle distribution were analyzed by flow cytometry （FCM）. The expression levels of phosphorylated extracellular signal-regulated kinase （p-ERK） and phosphorylated-Akt （p-Akt） proteins in A549 and H1299 cells were examined by Western blotting. Results： The proliferation of A54g and H1299 cells was inhibited via treatment with different concentrations of sorafenib in a dose-dependent manner （P〈O.05）. Sorafenib can induce apoptosis. The percentage of cells at G0/G1 phase was significantly increased, and which at S phase was significantly decreased in sorafenib-treated group, as compared with those in the control group （P〈0.05）. The expression level of p-ERK protein in A549 and H1299 cells treated with sorafenib was lower than that in the untreated control group （P〈0.05）. Conclusion： Sorafenib can inhibit the proliferation of human NSCLC A549 and H1299 cells and induce the apoptosis. This effect may be associated with the block of ERK signaling pathway.

关 键 词：癌 非小细胞肺 细胞外信号调节激酶类 细胞凋亡 信号传递 索拉非尼 

分 类 号：R734.2[医药卫生—肿瘤]