病毒性角膜炎的病原学诊断新法—多聚酶链反应  

A NEW METHOD TO DETECT PATHOGEN OF VIRAL KERATITIS-POLY MERASE CHAIN REACTION

作　　者：李晓亮[1] 王虎善[1] 宫枢政[1] 杨建凤[1] 孔媛 

机构地区：[1]成都军区昆明总医院眼科,昆明650032

出　　处：《西南国防医药》2000年第1期7-9,共3页Medical Journal of National Defending Forces in Southwest China

摘　　要：目的:评价多聚酶链反应技术对病毒性角膜炎病原学诊断的价值。方法:应用多聚酶链反应(Polymerase Chain Reaction PCR)技术检测12例患者患眼结膜囊分泌物中有无单纯疱疹病毒(Her-pes Simplex Virus,HSV)、巨细胞病毒(Cytomegaly Virus,CMV)或EB病毒(Estein-Barr Virus,EBV)的脱氧核糖核酸(DNA)片段。全部患者均临床诊断为病毒性角膜炎,其表现分为树枝状(6例),地图状(3例),盘状(1例),点状(2例)。结果:12例中PCR阳性者10例(9例检到HSV—DNA,1例检到CMV—DNA),阳性率为93.33％。结论:PCR是一种敏感、特异、快速、简便的病原学诊断方法,对病毒性角膜炎有早期诊断、鉴别诊断,以及指导治疗的价值。Objective: To assess value of polymerase chain reaction (PCR) on pathogenic diagnosis of viral keratitis. Methos: PCR was used to detect whether there were herpes simplex virus (HSV). cy-tomegaly virus (CMV) and Estein - Barr virus (EBV) DNA fragments in conjunctival sac secretion from 12 eyes of the patients who had been clinically classified into dendritic keratitis (6 ones), geographic keratitis (3 ones), disciform keratitis (one ) and punctate keratits (2 ones). Result: 10 of 12 eyes presented positive results. Among these 10 eyes, HSV - DNA was detected in 9 eyes and the other was CMV -DNA. Conclusion: PCR is not only sensitive and specific, but also rapid and convienient. It can be used as a new pathogenic diagnostic method of viral keratitis.

关 键 词：PCR 角膜炎 病毒感染 诊断 

分 类 号：R772.210.4[医药卫生—眼科]