一株拮抗可可球二孢菌放线菌的分离及鉴定  

Isolation and Identification of One Actinomycete Strain Exhibiting Antifungal Activity against Lasiodiplodia theobromae

作　　者：卞光凯[1] 缪倩[1] 秦盛[1] 陈永强[1] 曹成亮[1] 王祺琦[1] 陈凤美[1] 蒋继宏[1] 

机构地区：[1]徐州师范大学江苏省药用植物生物技术重点实验室,江苏徐州221116

出　　处：《生物技术》2011年第4期51-55,共5页Biotechnology

基　　金：国家自然科学基金项目(30872028;31000005);"十一五"科技支撑项目(2007BAD50B0204)资助

摘　　要：目的:筛选具有拮抗植物病原真菌可可球二孢菌活性的放线菌。方法:选择高氏一号培养基分离放线菌,以可可球二孢菌为指示菌进行抑菌活性筛选,通过形态学特征、生理生化特征、培养特征以及16S rRNA基因序列分析等研究对筛选得到的高活性菌株进行菌种鉴定。结果:从南方红豆杉根际土壤中筛选得到一株高活性菌株KLBMP 2284,16S rRNA基因序列比对结果显示其与弗吉尼亚链霉菌(Streptomyces virginiae)相似性98.963%,发酵液稀释300倍后抑制率为41.64%。结论:KLBMP 2284为弗吉尼亚链霉菌的一个菌株。Objective：To screen actinomycetes with anti-pathogenic fungi activity against Lasiodiplodia theobromae.Method：Actinomycetes were isolated by Gause I agar medium,then L.theobromae was selected to screen for the activity of anti-pathogenic fungi.The strain with high bioactivity was identified by the morphological,physiological,cultural characteristics and 16S rRNA gene sequence similarity analysis.Result：Strain KLBMP 2284 with high bioactivity was isolated from the rhizospheric soil of Taxus chinensis,similar to the Streptomyces virginiae with 16S rRNA gene sequence similarity value of 98.963%.After diluted 300 times,the inhibition rate of the fermentation broth against L.theobromae was 41.64%.Conclusion：Strain KLBMP 2284 was identified as the species of Streptomyces virginiae.

关 键 词：放线菌 可可球二孢菌 抑菌活性 鉴定 16S RRNA基因 

分 类 号：Q93-331[生物学—微生物学]