产纤维素酶黏细菌在生物转化的作用  

Myxobacterium Producing Cellulase in Conversion Lignocelluose to Ethanol

作　　者：王德韬[1] 贺燕云[1] 邱翔[1] 马中良[1] 

机构地区：[1]上海大学生命科学学院,上海200444

出　　处：《生物技术》2011年第4期78-82,共5页Biotechnology

基　　金：上海市高校选拔培养优秀青年教师科研专项基金项目(2009年)资助

摘　　要：目的:预处理对木质纤维素降解的影响。方法:从土壤中分离筛选到高纤维素酶活的黏细菌菌株So ce sh1008。该菌具有CMC酶活(CMCase)及微晶纤维素酶活性。研究NaOH联合黏细菌降解盐蒿、稻草、棉花秸秆和甘蔗渣四种木质纤维素的情况。结果:碱(2%NaOH)-黏细菌处理的方法优于黏细菌-碱的方法,其中降解棉花秸秆降解效果最明显,以5.0g木质纤维素为原料,其最终干重损失达2.1g,溶液中总糖含量和还原糖含量均值分别为12.8 mg/mL和0.93 mg/mL。酵母菌发酵产乙醇的研究结果表明,最佳发酵时间为47h,碱-黏细菌甘蔗渣降解液发酵效果最好,乙醇产出达6.0%。结论:黏细菌联合2%NaOH能有效降解甘蔗渣,提高乙醇产量。Objective：To investigate the effect of pretreatment methods on biomass.Method：Myxobacterium strains were isolated from soil by filter paper method,in which the strain So ce sh1008 with high cellulase activity was screened by Congo red.In this study,CMCase and microcrystalline cellulase activity of the strain were investigated.The hydrolysis of lignocellulose,namely artemisia stalk,rice straw,cotton stalk and bagasse via different pretreatments produced reducing sugar and total sugar.Result：The treatment with alkali（2% NaOH）-myxobacterium was superior to that with myxobacterium-alkali.The degradation of cotton stalk was obviously affected by alkali-myxobacterium in which the dry weight loss was 1.3g,while the average content of total sugar and reducing sugar was 12.8 mg/mL and 0.93 mg/mL,respectively.The optimum time of fermentation for ethanol was 47 hours,and the concentration can reach 6.0% in bagasse fermentation solution.Conclusion：Pretreatment of myxobacteria combined with 2%NaOH is a better way for degrading biomass.

关 键 词：黏细菌 木质纤维素 纤维素酶 生物转化 

分 类 号：TQ353.2[化学工程]