菊苣体细胞再生植株的遗传稳定性分析  

Genetic Stability Analysis about Somaclonal Regenerated Plants of Chicory

作　　者：张玉[1] 白史且[1] 李聪[2] 李达旭[1] 邓永昌[1] 王涌鑫[2] 

机构地区：[1]四川省草原科学研究院,四川成都611731 [2]中国农业科学院北京畜牧兽医研究所,北京100094

出　　处：《生物技术》2011年第5期51-54,共4页Biotechnology

基　　金：四川省应用基础项目(2008JY0010);四川省十二五科技攻关项目("牧草育种");国家十二五科技支撑计划项目(2011BAD17B03);国家牧草产业技术体系资助

摘　　要：目的:研究菊苣再生植株的遗传稳定性。方法:以菊苣叶片为外植体,经第一代和第二代体细胞再生获得再生植株,分别对再生植株提取DNA,从12个引物中筛选了2个多态性好的引物,对第一代和第二代体细胞再生植株进行RAPD标记。结果:菊苣第一代体细胞无性系没有发生DNA多态性变异,第二代体细胞无性系在分子水平发生了1条DNA多态性变异。结论:RAPD分子标记方法可以鉴定菊苣组织培养过程中的遗传稳定性和遗传变异,为菊苣快繁和遗传转化奠定了基础。Objective： To research the genetic stability of regenerated plants of chicory（Chicory intybus/ L.）.Method： Young leave of chicory plantlet in vitro was used as explants to induct regenerated plants by the first regeneration and the second filial regeneration.The DNA of the first regeneration and the second filial regeneration plants were extracted.2 good polymorphism primers were bolted from 12 primers,and the genetic stability of regenerated plants were analyzed with Randomly Amplified Polymorphic DNA（RAPD） markers.Result：The results indicated that somaclonal variation of the first regenerated plants were not found and somaclonal variation of the second filial regenerated plants was existed in DNA rnolecular level.Conclusion： It was indicated that RAPD markers could be applied successfully as a rapid,simple method for the establishment of genetic variation of donor parental field grown plants and the regeneration plants,which provided reference to rapid propagation in vitro and genetic translation.

关 键 词：菊苣 RAPD标记 遗传稳定性 体细胞变异 

分 类 号：O641[理学—物理化学]