赭曲霉的高密度培养对坎利酮转化的影响  

Effect of High Cell-Density Cultivation of Aspergillus ochraceus on Bioconversion of Canrenone

作　　者：刘晓[1] 刘逸寒[1] 别松涛[1] 徐星 谢进霞[1] 李建勋 韩开林[1] 路福平[1] 

机构地区：[1]工业发酵微生物教育部重点实验室工业酶国家工程实验室天津市工业微生物重点实验室天津科技大学生物工程学院,天津300457

出　　处：《生物技术》2011年第5期82-87,共6页Biotechnology

基　　金：国家"863"项目(2011AA02A211);天津市科技支撑计划重点项目(09ZCKFSH00600;10ZCKFSH00600)资助

摘　　要：目的:研究赭曲霉高密度培养的发酵培养基及条件,实现坎利酮的高转化。方法:选取廉价易得的培养基成分并进行优化,同时对发酵条件进行优化,得到了最优发酵培养基配方及培养条件。结果:发酵培养基最优配方为:葡萄糖20g/L,玉米浆20g/L,酵母膏20g/L,K2HPO4 2.5g/L。种子液最佳培养时间为24 h,发酵培养基初始pH 5.8,接种量为8%,装液量200mL/1 000mL,摇床转速为180 r/min,28℃,底物投料时间24 h,发酵结束时间72 h。结论:将该工艺在7 L发酵罐中放大,菌体密度达到25.36g/L,11α羟基坎利酮的转化率为86.1%。Objective：The optimum medium and culture conditions of Aspergillus ochraceus in high-density culture were studied to achieve the high bioconversion rate of canrenone.Method： We chose a kind of cheap and facile medium and optimized its ingredients and other fermentation conditions.Then we got optimum medium ingredients and culture conditions.Result： The optimum medium is： glucose 20g/L,corn steep liquor 20g/L,yeast extract 20g/L,K2HPO4 2.5g/L,other suitable fermentation conditions include： cultivation time of seed broth 24h,initial pH 5.8,inoculation amount 8%（V/V）,the liquid volume of 200 ml in 1 000 ml flask cultured at 28℃ on rotary shaker at 180 r/min for 72h,and the time of substrate addition at 24h.Conclusion： In 7 L fermentor,the density of cell reached 25.36g/L,and bioconversion rate of 11α-hydroxy-canrenone was 86.1%.

关 键 词：赭曲霉 高密度培养 11α羟基化坎利酮 转化率 

分 类 号：TQ920.6[轻工技术与工程—发酵工程] TQ467.8