H9N2亚型禽流感病毒基因组的遗传进化分析  

Genome Sequencing and Phylogenetic Analysis of Avian Influenza Viruses Subtype H9N2

作　　者：李树纯[1,2] 李心海[3] 仲书官[1,2] 孙化露[1,2] 潘金金[1,2] 陈素娟[1,2] 彭大新[1,2] 刘秀梵[1,2] 

机构地区：[1]扬州大学兽医学院 [2]扬州大学农业部畜禽传染病学重点开放实验室,扬州225009 [3]徐州生物工程职业技术学院,徐州221006

出　　处：《病毒学报》2012年第1期7-14,共8页Chinese Journal of Virology

基　　金：国家科技重大专项(2009ZX10004-214);973计划(2011CB505003);公益性行业科研专项(201003012);江苏自然科学研究重大项目(10KJA230055);青蓝工程;江苏高校优势学科建设工程资助项目

摘　　要：2009～2011年从江苏省、湖北省和安徽省等地来源于鸡、鸭、鹌鹑和鸽子的样品中分离鉴定出16株H9N2亚型禽流感病毒。通过反转录聚合酶链式反应(RT-PCR)扩增出分离株的全基因片段,并对其进行测序及遗传进化分析。序列分析显示,16株病毒HA基因裂解位点氨基酸序列为P-S-R/K-S-S-R,符合低致病性禽流感的分子特征;226位均为L,具有与哺乳动物唾液酸α,2-6受体结合的特性。M2基因均出现了对金刚烷胺产生耐药性的N31S突变。不同宿主来源的H9亚型AIV的主要分子特征一致。全基因遗传进化分析表明16株H9N2亚型禽流感病毒全基因发生了3配体重组,即以F98亚系AIV为骨架,HA来源于Y280亚系,PB2和M基因来源于G1亚系,形成了2种新的基因型。因此,要加强对H9N2亚型禽流感病毒的监测,密切关注它的重组趋势。Samples of chicken,duck,quail,and pigeon were collected from Jiangsu,Anhui,and Hebei in 2009-2011,and sixteen H9N2 subtype isolates of avian influenza virus（AIV） were identified.The eight full-length genes of 16 AIV isolates were amplified by RT-PCR and sequenced.Genome sequence analysis showed that the amino acid motif of cleavage sites in the HA gene was P-S-R/K-S-S-R,which was consistent with the characterization of the LPAIV,and the Leucine（L） at the amino acid position 226 in the HA genes of all isolates indicated the potential of binding with SAα,2-6 receptor.All isolates had a S to N substitution at residue 31 in the M2 gene,which is related to the resistance phenotype of adamantanes.The key molecular features of 16 AIV isolates from different hosts were same.Genome phylogenetic analysis revealed that all 16 H9N2 subtype AIVs originated from F98-like virus as backbone and formed two new genotypes through reassortment with HA gene of Y280-like virus and PB2 and M genes of G1-like virus.Our findings suggest that more attention should be paid to the surveillance of H9N2 influenza virus and its direction of reassortment.

关 键 词：H9N2 禽流感病毒 基因组 遗传进化分析 

分 类 号：R373.9[医药卫生—病原生物学] S852.64[医药卫生—基础医学]