他汀类药物减少神经细胞内β-淀粉样多肽的聚集  

Statins Reduce Intracellular Amyloid β Aggregation

作　　者：李晓晴[1] 王力[1] 王力锋[1] 

机构地区：[1]首都医科大学附属北京安贞医院神经科,北京100029

出　　处：《神经损伤与功能重建》2012年第1期9-12,共4页Neural Injury and Functional Reconstruction

基　　金：国家自然科学基金(No.30800350)

摘　　要：目的:探讨他汀类药物对β淀粉样多肽(Aβ)生成的影响。方法:将人神经母细胞瘤SH-SY5Y细胞分为对照组、转染组、阿托伐他汀干预组和辛伐他汀干预组,对照组不予任何处理,其它3组通过pcDNA3.0-YFP-C99质粒转染构建阿尔茨海默病(AD)细胞模型,阿托伐他汀干预组和辛伐他汀干预组分别加入不同浓度(5、10、20、50、100μmol/L)的阿托伐他汀、辛伐他汀。4组均在培养前和培养12、24、48、72h时,通过荧光显微镜定性观察,ELISA和Western blot定量分析细胞内Aβ的变化。结果:荧光显微镜下观察他汀类药物能显著减少细胞内Aβ聚集。不同浓度的他汀类药物和细胞培养时间均能减少Aβ,呈浓度-时间依赖性。他汀类药物浓度为20μmol/L和细胞培养24h时,各组间的Aβ减少有统计学意义(P<0.05)。结论:不同种类的他汀类药物能减少细胞内Aβ聚集,并且具有浓度-时间依赖性。Objective：To investigate the effect of Statins on intracellular Amyloid β（Aβ） aggregation.Methods： SH-SY5Y cells were divided into groups control,transfection,Atorvastatin and Simvastatin.The control group received no treatment,and the other 3 groups were transfected by plasmid pcDNA3.0-YFP-C99 to construct Alzheimer＇s disease（AD） cell models.The Atorvastatin group and Simvastatin group were added Atorvastatin and Simvastatin at different concentration（5、10、20、50、100 μmol/L） respectively.The Aβ changes in all the groups were observed with fluorescence microscope and quantitatively analyzed with ELISA assay and Western blot before and 12,24,48 and 72 h after culture.Results： In the pathogenic process of AD,A initially accumulated within the cells.By observation with fluorescence microscopy,intracellular Aβ was significantly reduced by statin treatment in a dose-and time-dependent manner.The Aβ reduction was statistically significant among the groups by 20 μmol/L statin treatment for 24 h（P0.05）.Conclusion： Aβ aggregation occurs initially in the AD cell model.Different types of statins could decrease Aβ accumulation in a dose-and time-dependent manner.

关 键 词：阿尔茨海默病 Β-淀粉样多肽 他汀类药物 

分 类 号：R741[医药卫生—神经病学与精神病学] R741.02[医药卫生—临床医学]