二甲基苯蒽诱发大鼠乳腺癌的病理及淋巴管生成的研究  

Study of Pathology and Lymphangiogenesis of DMBA Induced Rat Breast Cancer

作　　者：邹利光[1] 张松[1] 戚跃勇[1] 廖翠薇[1] 梁开运[1] 

机构地区：[1]第三军医大学新桥医院放射科,400037

出　　处：《实用癌症杂志》2012年第1期1-3,共3页The Practical Journal of Cancer

基　　金：国家自然科学基金(30770609;81071197)

摘　　要：目的研究大鼠诱发性乳腺癌发生的病理组织学和淋巴管密度(LVD)的变化。方法 Wistar雌性大鼠96只,二甲基苯蒽(DMBA)麻油溶液灌胃。16只大鼠9周内死亡,存活10周以上80只。从第10周开始至24周,每2周取10只活杀,乳腺肿块HE染色、podoplanin相关抗原免疫组化染色和免疫荧光染色。结果 75只大鼠成功诱发乳腺肿块,其中乳腺良性增生16只,炎性肉芽肿2只,乳腺癌57只。乳腺癌和乳腺增生组织LVD平均值分别为(6.93±0.84)、(1.52±0.23)个/高倍视野,乳腺癌组织LVD显著高于乳腺增生组织(P<0.01)。高分化、中分化和低分化乳腺癌LVD分别为(4.43±0.68)、(5.83±1.03)和(9.48±0.93)个/高倍视野,低分化乳腺癌LVD显著高于中、高分化乳腺癌(P<0.001)。结论 DMBA灌胃Wistar雌性大鼠乳腺癌诱发成功率高,肿瘤淋巴管密度与肿瘤分化程度密切相关。Objective To observe the histomorphological change and lymphatic vessel density（LVD） of rat induced breast cancer.Methods Ninety-six female Wistar rats were administrated intragastrically with 7,12-dimethyl-1,2-benzanthracene（DMBA） sesame oil solution.Sixteen rats died within 9 weeks.The remaining 80 rats were sacrificed from 10 to 24 weeks,10 rats every two weeks.Breast tumors were examined by Hematoxylin and eosin（HE） stain,podoplanin related antigen immunohistochemistry and immunofluorescence.Results Breast mass was successfully induced in 75 rats,of which 16 rats with benign hyperplasia,2 with inflammatory granuloma and 57 with breast cancer.LVD was（6.93±0.84） pieces/high power field（HPF） in breast cancer tissue,and（1.52±0.23） pieces/HPF in breast hyperplasia tissue.LVD of breast cancer was significantly higher than that of breast hyperplasia（P0.01）.LVD of well,moderately and poorly differentiated cancer was（4.43±0.68）,（5.83±1.03） and（9.48±0.93） pieces/HPF respectively.LVD of poorly differentiated cancer was significantly higher than that of well and moderately differentiated cancer（P0.001）.Conclusion Breast cancer can be induced in female Wistar rats successfully with DMBA.LVD of breast cancer is significantly associated with tumor differentiation degree.

关 键 词：大鼠 乳腺癌 淋巴管生成 

分 类 号：R737.9[医药卫生—肿瘤]