芬太尼对胃癌MGC-803细胞E2F-1、p53基因表达的影响  

Effects of fentanyl on the expression of E2F-1 and p53 genes of gastric carcinoma MGC-803 cells

作　　者：廖淳杰[1] 覃怡[1] 唐小曼[1] 谢玉波[1] 

机构地区：[1]广西医科大学第一附属医院麻醉科,南宁市530021

出　　处：《临床麻醉学杂志》2012年第1期63-64,共2页Journal of Clinical Anesthesiology

基　　金：国家自然科学基金(81160289);广西科学研究与技术开发计划项目(桂科攻1140003A-12);广西研究生教育创新计划(2009105981002M217)

摘　　要：目的观察芬太尼对人胃癌MGC-803细胞生长增殖及E2F-1、p53基因表达的影响。方法芬太尼10-4μmol/L与胃癌MGC-803细胞共同孵育为芬太尼组,未加入芬太尼的胃癌MGC-803细胞孵育为对照组。7d后检测胃癌MGC-803细胞生长增殖的变化,观察细胞超微结构的变化,检测细胞E2F-1、p53基因的表达。结果与对照组相比,芬太尼组MGC-803细胞生长增殖缓慢,细胞克隆形成率更低(P<0.05);细胞胞质深染、细胞核固缩、染色质碎裂,出现明显的凋亡表现;细胞E2F-1基因表达增加、p53基因表达减少。结论 10-4μmol/L芬太尼可抑制胃癌MGC-803细胞的生长增殖,促进细胞的凋亡,并改变E2F-1、p53基因的表达。Objective To observe the effects of fentanyl on the proliferation of human gastric carcinoma MGC-803 cells and the expression of E2F-1 and p53 genes. Methods MCK;-803 cells were incubated with fentanyl 10-4 μmol/L （fentanyl group） or not （control group）. Seven days later, the proliferation of MGC-803 cells was determined; the ultrastructure of MGC-803 cells was observed; and the expression of E2F-1 and p53 genes in MGC-803 cells was determined. Results Compared with control group, the proliferation of MGC-803 cells was slow with fewer cloning rate of MGC-803 cells in fentanyl group; morphological changes of apoptosis such as nuclear shrinkage and chromosomal DNA fragmentation were shown in fentanyl group; and E2F-1 mRNA upregulation and p53 mRNA downregulation were observed in fentanyl group. Conclusion Fentanyl 10-4 μmol/L may inhibit proliferation, promote apoptosis and alter the expression of E2F-1 and p53 genes of carcinoma MGC-803 cells.

关 键 词：芬太尼 胃癌 E2F-1 P53 

分 类 号：R735.2[医药卫生—肿瘤]