β-榄香烯对多发性骨髓瘤细胞的白细胞介素-6表达的影响  

Effects of elemene on the expression of interleukin-6 in human multiple myeloma cells

作　　者：郑旭[1] 关大刚[1] 佟海侠[1] 

机构地区：[1]中国医科大学附属盛京医院,110004

出　　处：《山西医药杂志（上半月）》2012年第2期107-109,共3页Shanxi Medical Journal

基　　金：国家自然科学基金(81101487);教育部归国留学回国人员科研启动基金(2011508);教育部高等学校博士学科点专项科研基金(20102104120014);辽宁省教育厅科研项目基金(L2010640)

摘　　要：目的探讨β-榄香烯对多发性骨髓瘤(MM)的白细胞介素-6(IL-6)表达的影响,以期了解β-榄香烯抗MM的分子机制。方法人MM细胞系RPMI 8226与不同浓度的β-榄香烯作用,以酶联免疫吸附试验(ELISA)法检测MM细胞培养上清中IL-6的浓度,逆转录聚合酶链反应(RT-PCR)检测IL-6基因表达。结果不同浓度的β-榄香烯作用RPMI 8226细胞48h后,培养上清中IL-6浓度与对照组相比,差异有统计学意义(P<0.01)。RT-PCR检测结果表明,不同浓度的β-榄香烯作用于RPMI 8226细胞48h,RPMI 8226细胞的IL-6基因mRNA表达强度与对照相比均明显降低。结论β-榄香烯能明显减少MM细胞IL-6的自分泌,抑制RPMI 8226细胞的IL-6基因表达。提示β-榄香烯降低IL-6表达可能是其抗MM的机制之一。Objective To investigate the effects of β-elemene on the expression of interleukin（IL）-6 of in human multiple myeloma（MM） cells.Methods In order to evaluate the concentrations of IL-6 and the gene expression of IL-6,the supernatant of RPMI 8226 cells were measured by ELISA and RT-PCR methods after treated with different concentrations of β-elemene.Results The concentrations of IL-6 on the supernatant of RPMI 8226 cells were significantly decreased compared with control.The expression of IL-6 gene were significantly reduced after treatment with β-elemene.Conclusion β-elemene can significantly inhibit the secretion and the expression of IL-6 in RPMI 8226 cells,which seems to be one of mechanisms of anti-MM effects of β-elemene.

关 键 词：多发性骨髓瘤 白细胞介素6 Β-榄香烯 

分 类 号：R733.3[医药卫生—肿瘤]