UPLC测定普洱茶饮料中咖啡因的含量  

Determination of content of caffeine in Pu-erh tea drinks by UPLC

作　　者：张毅[1] 高展[1] 王蕊[1] 孙玉侠[1] 曹凤兰[1] 

机构地区：[1]天津天士力制药股份有限公司质检中心,天津300410

出　　处：《饮料工业》2012年第1期41-44,共4页Beverage Industry

摘　　要：目的:使用UPLC快速测定普洱茶饮料中咖啡因的含量。方法:色谱柱为ACQUITY UPLC HSST3(50×2.1mm,1.8μm);流动相为甲醇-水(20:80);流速为0.4ml/min;紫外检测波长为271nm。结果:该方法操作简便,测定准确,浓度的线性关系良好(r=0.9999),加样回收率为99.36%～100.06%(RSD=0.25%)。结论:该方法简单、快速、准确,结果令人满意。Objective：To quickly determine the content of caffeine in Pu-erh tea drinks by the Ultra Performance Liquid Chromatography（UPLC） method.Method：The column was ACQUITY UPLC HSS T3（50 × 2.1 mm,1.8 μm）,the mobile phase was methanol-water（20：80）,the flow was 0.4 ml/min,and the wavelength 271 nm.Results：Being simple and accurate,the method had a good linear relationship with concentration（r = 0.9999）,and the recoveries ranged from 99.36% to 100.06%.Conclusion：The method was fast,simple and accurate,and the results were satisfactory.

关 键 词：咖啡因 普洱茶饮料 UPLC 含量 

分 类 号：TS275.2[轻工技术与工程—农产品加工及贮藏工程] O657.72[轻工技术与工程—食品科学与工程]