结直肠癌中CDH13基因甲基化及其临床意义  

Methylation of CDH13 gene in colorectal cancer and its clinical significance

作　　者：王丽[1] 汪竹[1] 袁昕[1] 焦南林[2] 张有为[1] 童建东[1] 

机构地区：[1]扬州市第一人民医院肿瘤科,江苏省225009 [2]皖南医学院附属弋矶山医院病理科

出　　处：《江苏医药》2012年第2期199-201,共3页Jiangsu Medical Journal

摘　　要：目的探讨H-钙黏素(CDH13)基因在结直肠癌(CRC)中的甲基化状态及其临床意义。方法选取85例CRC患者组织及其相应正常组织,显微切割结合甲基化特异性聚合酶链反应检测CDH13基因启动子区域甲基化。结果 85例CRC组织中,27例(31.8%)检出CDH13基因甲基化,显著多于相应正常组织的8例(9.4%)(P<0.01)。CRC组织中CDH13基因异常甲基化与肿瘤低分化有关(P<0.05),并且在进展期及淋巴结转移的患者中显示出一定优势。CDH13甲基化与不良总生存(OS)有关(P<0.01),是其独立预后指标。结论 CDH13基因甲基化有可能成为CRC预后判断的新型分子标记。Objective To investigate the methylation status of H-cadherin（CDH13） gene in colorectal cancer（CRC） and its clinical significance.Methods Eighty-five CRC specimens and tumor-adjacent normal tissues were selected,and the methylation status of CDH13 gene in the promoter region was detected by manual microdissection combined with methylation-specific PCR.Results The methylation frequency of CDH13 in the CRC tissues was 31.8%（27/85）,which was significantly higher than 9.4%（8/85） in the tumor-adjacent normal tissues（P〈0.01）.The methylation of CDH13 gene in the CRC tissues was associated with the poor differentiation（P〈0.05） and tended to be predominant in the advanced stages and lymph metastasis.Moreover,the methylation level of CDH13 gene was correlated with adverse overall survival（OS）（P〈0.01） and was an independent prognostic factor for OS.Conclusion The methylation of CDH13 gene may be a new biomarker for predicting the prognosis of CRC.

关 键 词：结直肠癌 H-钙黏素 甲基化 

分 类 号：R735[医药卫生—肿瘤]