Ultrasound microbubbles combined with liposome-mediated pNogo-R shRNA delivery into neural stem cells  

作　　者：Weixia Ye Xueping Huang Yangyang Sun Hao Liu Jin Jiang Youde Cao 

机构地区：[1]Department of Pathology, Molecular Medicine and Cancer Research Center, Chongqing Medical University, Chongqing 400016, China [2]Department of Orthopaedics, Luzhou People＇s Hospital, Luzhou 646000, Sichuan Province, China

出　　处：《Neural Regeneration Research》2012年第1期54-59,共6页中国神经再生研究（英文版）

基　　金：supported by the Natural Science Foundation of Chongqing (Ultrasound microbubble combining with NgR-RNAi promoted neural stem cells repair of spinal cord injury in rats), No. 2008BB5223

摘　　要：In the present study, ultrasound-mediated microbubble destruction （UMMD） alone and combined with liposome technology was used as a novel nonviral technique to transfect a Nogo receptor （Nogo-R） shRNA plasmid （pNogo-R shRNA） into neural stem cells （NSCs）. Using green fluorescent protein as a reporter gene, transfection efficiency of NSCs was significantly higher in the group transfected with UMMD combined with liposomes compared with that of the group transfected with UMMD or liposomes alone, and did not affect cell vitality. In addition, Nogo-R mRNA and protein expression was dramatically decreased in the UMMD combined with liposome-mediated group compared with that of other groups after 24 hours of transfection. The UMMD technique combined with liposomes is a noninvasive gene transfer method, which showed minimal effects on cell viability and effectively increased transfer of Nogo-R shRNA into NSCs.In the present study, ultrasound-mediated microbubble destruction （UMMD） alone and combined with liposome technology was used as a novel nonviral technique to transfect a Nogo receptor （Nogo-R） shRNA plasmid （pNogo-R shRNA） into neural stem cells （NSCs）. Using green fluorescent protein as a reporter gene, transfection efficiency of NSCs was significantly higher in the group transfected with UMMD combined with liposomes compared with that of the group transfected with UMMD or liposomes alone, and did not affect cell vitality. In addition, Nogo-R mRNA and protein expression was dramatically decreased in the UMMD combined with liposome-mediated group compared with that of other groups after 24 hours of transfection. The UMMD technique combined with liposomes is a noninvasive gene transfer method, which showed minimal effects on cell viability and effectively increased transfer of Nogo-R shRNA into NSCs.

关 键 词：ultrasound microbubble LIPOSOME neural stem cell gene transfection Nogo receptor neural regeneration 

分 类 号：Q421[生物学—神经生物学] Q522[生物学—生理学]