乙二醛酶Ⅰ介导孕激素对子宫内膜癌细胞增殖和凋亡活性的调控  

Progestin-induced proliferative and apoptotic activity in endometrial cancer cell mediated by Glyoxalase Ⅰ

作　　者：王倩倩[1] 廖虹[2] 魏桠楠 吴凌[4] 赖允丽[1] 孙姗姗[1] 赵贵旭 顾红琴 祝亚平[1] 张箴波[1] 丰有吉[1] 

机构地区：[1]上海交通大学附属上海市第一人民医院妇产科,上海200080 [2]上海市第一妇婴保健院,上海200040 [3]山东省滕州市中心医院妇产科,滕州277500 [4]厦门大学附属第一人民医院,厦门361002

出　　处：《现代妇产科进展》2012年第1期12-16,共5页Progress in Obstetrics and Gynecology

基　　金：国家自然科学基金(No:81001155;81020108027);上海市卫生局面上项目(No:2009028)

摘　　要：目的:探讨孕激素是否通过其受体调控乙二醛酶Ⅰ(GloⅠ)的表达,进而调控子宫内膜癌细胞的增殖和凋亡活性。方法:应用Westen blot检测孕激素对GloⅠ,Caspase3,Cyclin D1表达的影响;经孕激素受体(PR)特异性抑制剂RU-486处理后,用Westen blot检测PR介导的GloⅠ,Caspase3,Cyclin D1分子的表达;应用siRNA干扰技术敲除GloⅠ表达后,检测GloⅠ对caspase3和Cyclin D1表达的影响,并分别应用MTT和TUNEL检测孕激素通过GloⅠ介导对细胞增殖和凋亡的影响。结果:(1)孕激素呈剂量依赖效应地下调GloⅠ、Cyclin D1的表达、上调Caspase3的表达,与对照组相比差异有统计学意义(P<0.05);(2)MPA+RU-486组GloⅠ,Cyclin D1的蛋白水平比RU-486组、MPA处理组增加,Caspase3的表达下降,差异有统计学意义(P<0.05);(3)siGloⅠ干扰组GloⅠ蛋白表达水平比阴性对照组明显降低,干扰效率达50%(P<0.05),GloⅠ被干扰后,加入孕激素细胞的增殖活性明显受到抑制,凋亡活性增加,差异有统计学意义(P<0.05)。结论:孕激素可通过PR调控GloⅠ介导的子宫内膜癌细胞增殖和凋亡活性。Objective：To investigate whether MPA regulates the expession of GloⅠ through the progesterone receptor,following regulates endometrial cancer cell proliferation and apoptotic activity.Methods：Westen blot was used to detect the expression of GloⅠ,Caspase3,Cyclin D1 in protein levels after treatment with the progesterone;After the treatment of PR specific inhibitor,Westen blot was used to detect the expression of GloⅠ,Caspase3,Cyclin D;siRNA was used to konck down the expression of GloⅠ gene and MTT assay was used to determine cellular growth,TUNEL was used to determine cellular apoptosis.Results：（1）The expression of GloⅠ,Cyclin D1 could be down-regulated by MPA in Ishikawa cells and the expression of Caspase3 could be up-regulated by MPA in dose-dependent（P0.05）.（2）The expression of GloⅠ,Cyclin D1 was up-regulated and the Caspase3 was down-regulated by MPA±RU-486 compared with control group（P0.05）.3.Inhibition of GloⅠ by RNA interference could reduce cell proliferation and increase cell apoptosis（P0.05）.Conclusion：MPA can regulate cell proliferation and apoptosis activity by GloⅠ gene through the progesterone receptor.

关 键 词：子宫内膜癌 甲羟孕酮 孕激素受体 乙二醛酶Ⅰ 细胞增殖和凋亡 

分 类 号：R737.33[医药卫生—肿瘤]