GnRH-a对子宫内膜异位症腹膜纤溶相关因子tPA/PAI-1表达影响的研究  

Study on the expression of tPA and PAI-1 in endometriosis peritoneum treated by GnRH-a

作　　者：李孟慧[1] 冷金花[1] 李成龙[1] 史精华[1] 贾双征[1] 郎景和[1] 

机构地区：[1]中国医学科学院北京协和医学院北京协和医院协和转化医学中心妇产科,北京100730

出　　处：《现代妇产科进展》2012年第1期20-22,26,共4页Progress in Obstetrics and Gynecology

基　　金：国家自然科学基金项目(No:30950020)

摘　　要：目的:探讨子宫内膜异位症(EM)腹膜组织纤溶相关因子表达水平及使用促性腺激素释放激素类似物(GnRH-a)后表达的变化。方法:应用免疫组织化学方法检测组织型纤溶酶原激活物(tPA)、纤溶酶原激活物抑制剂(PAI)-1、尿激酶纤溶酶原激活物(uPA)在内异症和正常对照组腹膜组织中的表达。分析GnRH-a对各种纤溶相关因子表达的影响。结果:内异症腹膜组织中纤溶相关因子tPA、PAI-1、uPA表达均高于对照组腹膜组织的相应表达,差异有统计学意义(P<0.01)。应用GnRH-a的EM组tPA表达水平明显升高,且PAI-1表达水平降低,差异有统计学意义(P<0.05),GnRH-a应用对uPA表达无明显影响。结论:与正常腹膜相比内异症腹膜组织纤溶相关因子tPA、PAI-1、uPA表达水平发生变化,纤溶活性的变化可能是腹膜对损伤发生的反应。GnRH-a应用使tPA表达升高、PAI-1表达降低,发挥促进纤溶作用,从而抑制腹膜粘连形成。Objective： To investigate the expression levels of fibrinolytic factors in the endometriosis（EM） peritoneum and the different expressions after treated by GnRH-a.Method： Detect the expression levels of tPA,PAI-1 and uPA of peritoneum from EM group and control group by immunohistochemistry methods.Investigate the effect of gonadotropin-releasing hormone analogue（GnRH-a） on the expression of those fibrinolytic factors.Results： The levels of tPA,PAI-1 and uPA from peritoneum of EM group were higher than those in the control group and there was statistically significant differences（P0.01）.GnRH-a could up-regulate tPA levels,down-regulate PAI-1 and the difference was statistically significant（P0.05）,but GnRH-a had no effect on uPA.Conclusions：Compared to the control group,EM group have the up-regulating of tPA,PAI-1,uPA,which may be the response of peritoneum to injuries;GnRH-a may promote fibrinolysis by regulating fibrinolytic factors,such as increasing tPA and decreasing PAI-1,and then inhibit the peritoneal adhesion formation.

关 键 词：子宫内膜异位症 腹膜 纤溶因子 促性腺激素释放激素类似物 

分 类 号：R737.33[医药卫生—肿瘤]