宫颈病变细胞中hTERC、c-myc基因的表达及临床意义  

Expression and clinical significance of hTERC gene,c-myc gene in cytological specimens of cervix

作　　者：武海英[1,2] 史惠蓉[1] 孔令非[2] 何伟[3] 

机构地区：[1]郑州大学第一附属医院妇产科,郑州450052 [2]河南省人民医院妇产科,郑州450003 [3]郑州大学继续教育学院,郑州450052

出　　处：《现代妇产科进展》2012年第1期47-50,共4页Progress in Obstetrics and Gynecology

摘　　要：目的:检测各级别宫颈上皮内瘤变、宫颈癌患者端粒酶(hTERC)和c-myc基因的表达,探讨二者与宫颈癌发生发展的相关性。方法:采用FISH技术检测193例宫颈病变及52例宫颈癌患者中hTERC基因,c-myc基因的扩增情况,比较分析两种基因表达与CIN级别及宫颈癌临床病理特征的关系。结果:随宫颈病变级别加重,hTERC基因阳性扩增率呈逐渐增高趋势,组间有显著统计学差异(χ2=148.353,P=0.000)。c-myc基因随宫颈病变级别的加重,阳性率逐渐增高,组间有显著统计学差异(χ2=110.128,P=0.000)。c-myc基因阳性率随分化程度的加重而升高,G3阳性率明显高于G1/G2(χ2=6.054,P=0.014),hTERC基因则无显著差异。结论:hTERC基因,c-myc基因可作为鉴别宫颈低度癌前病变和高度癌前病变的重要辅助指标,c-myc基因可能是揭示宫颈癌进展,分化程度的重要指标。Objective： To detect the expression of hTERC gene and c-myc gene in the different grades of cervical intraepithelial neoplasia and cervical cancer patients and investigate their relationship with cervical cancer.Methods： Using fluorescence in situ hybridization（FISH） to detect the amplification of hTERC gene and c-myc gene in cytological specimens and to compare their expression with clinical pathological characters in 193 cervical lesions and 52 cervical cancers.Results： The amplification of hTERC gene increased with the increased level of cervical lesions.There was statistically significant difference between each group（χ2=148.353,P=0.000）.c-myc gene expression showed the same pattern（χ2=110.128,P=0.000）.G3 positive rate of c-myc gene was significantly higher than G1/G2（χ2 = 6.054,P= 0.014） with the increased level of cervical lesions while there was no significant difference for hTERC gene.Conclusions： hTERC gene c-myc gene can be used as important supplementary indicators of differential diagnosis for low-grade and high-grade cervical precancerous lesions.c-myc gene may be an important indicator for the development and differentiation of cervical cancer.

关 键 词：宫颈肿瘤 荧光原位杂交技术 端粒酶 基因 C-MYC 

分 类 号：R737.33[医药卫生—肿瘤]