去肾神经对中年自发性高血压大鼠血压的影响  

Renal denervation in treatment of spontaneous hypertension in adult rats

作　　者：刘洁[1] 周音频[1] 王霄[1] 杨易[1] 黄岚[1] 

机构地区：[1]第三军医大学新桥医院全军心血管内科研究所,重庆400037

出　　处：《第三军医大学学报》2012年第4期336-340,共5页Journal of Third Military Medical University

基　　金：第三军医大学临床科研基金(2009XLC16)~~

摘　　要：目的研究去肾神经对中年自发性高血压大鼠血压的影响。方法 30周龄自发性高血压大鼠(spontaneous hypertensive rats,SHR)及正常血压大鼠(Wistar kyoto rats,WKY)各25只,按随机区组设计分为5组,根据观察时间分为1、4、9、16、32 d组(n=10),于第2天行去肾神经术。无创鼠尾血压仪(BP-98A)测量血压;10%苯酚乙醇溶液去肾神经;ELISA法测定肾组织中去甲肾上腺素(nonadrenaline,NE)含量及肾素活性;RT-PCR法和Western blot法测定大鼠腹主动脉α1、β1-肾上腺素能受体(α1、β1-受体)在mRNA和蛋白水平上的表达水平。结果未去肾神经的中年SHR血浆及肾组织NE含量及肾素活性水平明显高于中年WKY(P<0.05),主动脉α1、β1-受体mRNA和蛋白的表达与同龄的WKY差异无显著性(P>0.05);去肾神经急性期,中年SHR血压迅速下降(P<0.05),血浆与肾组织NE含量及肾素活性降低(P<0.05);主动脉α1-受体mRNA和蛋白表达上调(P<0.05),β1-受体mRNA和蛋白表达差异无显著性(P>0.05);慢性期,肾组织NE含量和肾素活性升高(P<0.05),主动脉α1、β1-受体mRNA和蛋白表达均上调(P<0.05)。去肾神经对中年WKY无显著性影响(P>0.05)。结论去肾神经对于中年SHR大鼠的降压效应是暂时的,急性期血压下降,α1-受体表达上调,β1-受体表达无变化;慢性期血压升高,α1、β1-受体表达均上调;血压的变化与NE及肾素活性的变化一致。Objective To determine the effect of renal denervation on hypertension in the middle-aged rats.Methods A total of 25 spontaneous hypertension rats（SHR） and 25 Wistar kyoto rats（WKY） aging 30-week-old male rats,weighing 350 to 420 g were randomly divided into 5 groups randomly,that is,1-day group,4-day group,9-day group,16-day group,and 32-day group（n=10,including 5 SHR and 5 WKY rats in each group）.The implementation of the renal denervation was on the second day with 10% phenol ethanol solution.Blood pressure was measured by a non-invasive tail blood pressure meter（BP-98A）.The plasma and renal tissue norepinephrine（nonadrenaline,NE） content and rennin activity were detected by ELISA.The expression of α1-and β1-adrenoreceptor（α1-R and β1-R）in rat aortic smooth muscle were determined by RT-PCR and Western blotting respectively for their mRNA and protein levels.Results NE content and renin activity in the plasma and renal tissue were significantly higher in middle-aged SHR than in middle-aged WKY rats（P0.05）.However,the expression of the aorta α1-R and β1-R at the mRNA and protein levels had no significant difference in middle-aged SHR and WKY rats（P0.05）.Rapid decline in blood pressure was observed in middle-aged SHR after denervation（P0.05）,and NE content and renin activity in the plasma and renal tissue was significantly decreased（P0.05）,but the expression of the aorta α1-R was up-regulated（P0.05）,and β1-R had no difference（P0.05）.In the chronic denervation period,the blood pressure as well as renal NE content and renin activity were gradually higher than normal levels（P0.05）.The expression of α1-R and β1-R were up-regulated which ultimately higher than initial levels（P0.05）.The expression of α1-R and β1-R protein was similar with the expression of mRNA.Conclusion Renal denervation attenuates the high blood pressure temporarily in the middle-aged SHR.During the acute period,α1-R is up-regulated.However,blood pressure is gradually increased in c

关 键 词：去肾神经 高血压 大鼠 近交SHR 中年 

分 类 号：R-332[医药卫生] R544.1