PJ34对卵巢癌C13~＊细胞生长活性及耐药性的影响  

Effects of PJ34 on growth activity and cisplatin resistance of ovarian cancer C13~＊ cells

作　　者：张敬敬[1] 王哲[1] 田永杰[1] 张捷[2] 

机构地区：[1]山东大学附属省立医院妇产科,济南250021 [2]山东大学附属省立医院中心实验室,济南250021

出　　处：《山东大学学报（医学版）》2012年第1期46-50,共5页Journal of Shandong University:Health Sciences

基　　金：山东省自然基金资助项目(ZR2009CM104);山东省医药卫生科技发展计划项目(2009HZ065)

摘　　要：目的探讨聚腺苷酸二磷酸核糖聚合酶-1[Poly(ADP-ribose)polymerase-1,PARP-1]抑制剂PJ34对卵巢癌耐药细胞株C13*细胞生长活性及耐药性的影响。方法应用四甲基偶氮唑蓝(MTT)及流式细胞术检测PJ34对C13*细胞顺铂敏感性、凋亡和周期的影响;免疫荧光及蛋白印迹法(Western blot)检测PJ34处理前后PARP-1的表达情况。结果 C13*细胞增殖抑制率及对顺铂的敏感性随顺铂及PJ34浓度的增加而增强(P<0.05);细胞凋亡率随PJ34浓度的增加而增高(P<0.05);周期为PJ34浓度越高,G0/G1期细胞越多,S期细胞越少;PJ34可下调细胞中PARP-1的表达,顺铂与PJ34合用后,PARP-1蛋白表达量明显降低(P<0.01)。结论 PJ34能显著下调C13*细胞内PARP-1表达,抑制细胞增殖,促进细胞凋亡,增加细胞对顺铂的敏感性,有效逆转C13*细胞对顺铂的耐药性。Objective To investigate the effects of poly（ADP-ribose）polymerase-1（PARP-1） inhibitor PJ34 on the growth activity and cisplatin resistance of cisplatin-resistant ovarian cancer C13＊ cells in vitro.Methods MTT assay and flow cytometry were used to determine influences of PJ34 on cisplatin sensitivity,apoptosis and the cell cycle of C13＊ cells.The expression level of PARP-1 in C13＊ cells was determined using immunofluorescence microscopy and Western blot,and changes induced by PJ34 treatment were investigated.Results The inhibitory ratio and cisplatin sensitivity of C13＊ cells were significantly increased with increases of concentrations of platinum（Pt） and PJ34（P0.05）.The apoptosis ratio of C13＊ cells was significantly increased with the increase of the concentration of PJ34（P0.05）.The proportion of cells in the G0/Gl phase in the high concentration PJ34-treated group was larger than that in the low concentration PJ34-treated group.PJ34 down-regulated the PARP-1 expression in C13＊ cells,which was remarkably decreased when Pt and PJ34 co-treated（P0.01）.Conclusion PJ34 remarkably down-regulates the PARP-1 protein expression,inhibits proliferation activity,promotes apoptosis,and increases sensitivity to cisplatin of C13＊ cells.Hopefully,PJ34 could effectively reverse resistance of C13＊cells to cisplatin.

关 键 词：聚腺苷二磷酸核糖聚合酶-1 PJ34 卵巢癌 耐药性 

分 类 号：R737.31[医药卫生—肿瘤]