膀胱尿路上皮癌中miRNA-300的差异表达  

Expression of miRNA-300 in Urothelial Carcinoma of the Bladder

作　　者：徐锋[1] 高建平[1] 张征宇[1] 葛京平[1] 位志峰[1] 程文[1] 

机构地区：[1]南京军区南京总医院泌尿外科,210002

出　　处：《医学研究杂志》2012年第1期39-42,共4页Journal of Medical Research

基　　金：国家自然科学基金资助项目(30772278);南京军区南京总医院青年课题(2009-2011)

摘　　要：目的探讨miRNA-300在膀胱尿路上皮癌中的差异表达与其生物学行为的关系。方法常规提取30例膀胱尿路上皮癌及5例正常膀胱黏膜组织中总RNA,应用荧光定量RT-PCR方法检测标本中miRNA-300的表达情况。结果与正常膀胱黏膜上皮组织相比,膀胱尿路上皮癌中miRNA-300表达明显下调(P<0.01)。结论 miRNA-300的差异表达极可能为促进膀胱尿路上皮癌的发生及发展因素之一。To investigate the gene expression disparity and its relationship with biological behavior of miRNA - 300 in urothelial carcinoma of the bladder （BUC）. Methods Thirty BUC and five normal bladder mucosa tissues were collected and the total RNA was extracted routinely. The miRNA - 300 expression was detected by using real - time quantitative polymerase chain reaction. Re- sults The expression of miRNA - 300 was significantly down - regulated in BUC tissues than those in normal bladder mucosa. Conclu-sion miRNA -300 may Participate in the pathogenesis and development of BUC.

关 键 词：miRNA-300膀胱尿路上皮癌 实时荧光定量 

分 类 号：R737.14[医药卫生—肿瘤]