慢病毒载体介导RNA干扰对乳腺癌MDA-MB-231细胞LOX基因表达的影响  

EFFECTS OF LOX GENE EXPRESSION ON BREAST CANCER CELL LINE MDA-MB-231 BY LENTIVIRAL VECTOR-MEDIATED RNA INTERFERENCE

作　　者：李景涛[1] 刘剑仑[1] 韦薇[1] 杨华伟[1] 

机构地区：[1]广西医科大学附属肿瘤医院乳腺外科,南宁530021

出　　处：《广西医科大学学报》2011年第6期834-836,共3页Journal of Guangxi Medical University

基　　金：广西科学研究与技术开发计划课题(No.桂科攻0816004-14)

摘　　要：目的:观察慢病毒干扰载体(LOX-RNAi-LV)转染对乳腺癌高侵袭转移潜能MDA-MB-231细胞LOX基因表达的影响。方法:设计合成特异性的LOX RNA干扰慢病毒载体,稳定转染至MDA-MB-231细胞中,实时定量PCR、Western blot检测LOX mRNA和蛋白的表达情况。结果:稳定转染慢病毒RNAi后,60h后有GFP荧光表达,72h后表达明显增强,转染率达90%;LOX mRNA和蛋白的表达被显著抑制,抑制率分别为89.2%和83%。结论:稳定转染慢病毒干扰载体(LOX-RNAi-LV)可有效抑制乳腺癌细胞MDA-MB-231中LOX mRNA和蛋白表达。Objective：To explore lysyl oxidase（LOX） silencing by Lentiviral transfection on the breast cancer cell line MDA-MB-231.Methods：Base on the sequence of LOX,lentiviral RNA interference vector was designed and chemically synthesized,and then transfected into the breast cancer cell line MDA-MB-231.The expression of LOX mRNA and protein was detected by Real-time fluorogenic quantitative PCR（real-time PCR） and Western blot.Results：After the stable transfection,the expression of LOXmRNA and protein was significantly inhibited.Conclusion：Stable transfection of lentiviral vector interference LOX-RNAi-LV can significantly inhibit the expression of LOX mRNA and protein.

关 键 词：乳腺癌 赖氨酰氧化酶 RNA干扰 

分 类 号：R737.9[医药卫生—肿瘤]