Effect of Quercetin on the Proliferation and Mitochondrial Transmembrane Potential of CBRH-7919 Cells  

槲皮素对CBRH-7919细胞增殖及线粒体膜电位的影响(英文)

作　　者：马朋[1] 曹同涛[2] 于敏[1] 闫苗苗[1] 牛新华[1] 

机构地区：[1]滨州医学院药学院,山东烟台264003 [2]滨州医学院中西医结合学院,山东烟台264003

出　　处：《Agricultural Science & Technology》2012年第2期245-247,共3页农业科学与技术（英文版）

基　　金：Supported by the Natural Science Foundation of Shandong Province(ZR2011CM021);the Science and Technology Plan of Binzhou Medical University(BY2010KJ087)~~

摘　　要：[Objective] To investigate the effect of quercetin on the proliferation and mitochondrial transmembrane potential of CBRH-7919 cells. [Method] The CBRH-7919 cells of hepatocarcinoma were cultured in vitro. After treated with different concentrations of quercetin, the OD405 nm of CBRH-7919 cells was detected by using the acid phosphatase assy （APA）; morphologic changes of the cells were observed under inverted microscope; the mitochondrial transmembrane potential （△ψm） intensity changes of CBRH-7919 cells were analyzed by flow cytometry after stained with Rhodamine 123. [Result] Quercetin inhibited the proliferation of CBRH-7919 cells significantly, and the growth inhibitory effect presented time- and dose-dependent relationship. Typical decrease of cell density was observed by optical microscopy on the quercetin-treated cells. With the effect of 10 μg/ml quercetin on CBRH-7919 cells for 12, 24 and 48 h, the percentage of Rhodamine 123 stained hypofluorescence cells increased, while the mitochondrial transmembrane potential（△ψm） intensity of CBRH-7919 cells decreased. [Conclusion] Quercetin could inhibit the proliferation of CBRH-7919 cells in vitro, causing the decrease in mitochondrial transmembrane potential.[目的]探讨槲皮素对肝癌细胞株CBRH-7919增殖及线粒体膜电位的影响。[方法]体外培养肝癌CBRH-7919细胞,以不同浓度槲皮素处理细胞后,采用酸性磷酸酶(APA)法检测OD405nm;倒置显微镜观测CBRH-7919细胞形态变化;Rhodamine123染色后流式细胞仪检测线粒体膜电位(△ψm)变化。[结果]槲皮素对肝癌CBRH-7919细胞有明显的增殖抑制作用,并与剂量和时间成正比。光学显微镜下可见槲皮素作用后细胞密度明显降低。经10μg/ml槲皮素作用12、24、48h后,流式细胞术检测到Rhodamine123染色弱荧光细胞百分比逐步增加,线粒体膜电位下降。[结论]槲皮素体外能抑制肝癌细胞株CBRH-7919增殖,引起线粒体膜电位下降。

关 键 词：QUERCETIN HEPATOCARCINOMA Mitochondrial transmembrane potential 

分 类 号：R285[医药卫生—中药学] R735.7[医药卫生—中医学]