离子液体和β-环糊精修饰反向微乳毛细管电动色谱分析化妆品中的激素  

Analysis of corticosteroids in cosmetics by reversed microemulsion electrokinetic chromatography with 1-Butyl-3-methylimizolium tetrafluoborate ionic liquid and β-cyclodextrin

作　　者：李萌[1] 金勉勉[1] 李娟 陈新 商少明 刘瑛 毛明星 

机构地区：[1]无锡出入境检验检疫局,无锡214101 [2]江南大学化学与材料工程学院,无锡214122

出　　处：《分析试验室》2012年第2期75-78,共4页Chinese Journal of Analysis Laboratory

基　　金：国家质检总局项目(2010IK183)资助

摘　　要：建立了以离子液体和β-环糊精为添加剂的反向微乳毛细管电泳(MEEKC)法分离测定化妆品中丙酸氟替卡松、曲安奈德、醋酸可的松、地塞米松、氢化可的松和泼尼松6种激素的方法。在优化的实验条件下,6种物质于12min内达到基线分离,丙酸氟替卡松在2.0～200mg/L范围内线性关系良好,其它组分在2.0～500mg/L范围内线性关系良好,检出限(S/N=3)分别为0.75、0.81、0.66、0.68、0.74、0.86 mg/L。方法用于化妆品样品测定,加标回收率在93.5%～105%之间,RSD均小于4.3%(n=3)。A reversed microemulsion electrokinetic chromatographic（MEEKC） method was developed for the separation of six corticosteroids（fluticasone propionate,triamcinolone acetonide,cortisone acetate,dexamethasone,hydrocortisone and prednisone） by using 1-butyl-3-methylimizolium tetrafluoborate（BMIM-BF4） ionic liquid（IL） and β-CD as additives.The effect of experimental conditions,such as BMIM-BF4 concentration and pH value on the separation efficiency were investigated.Under the optimized conditions,six analytes could be separated within 15 min,the calibration plot of fluticasone propionate was linear in the range of 2.0-200mg/L and the rest were linear in the range of 2.0-500mg/L,the detection limits（S/N=3） for the six corticosteroids were 0.75,0.81,0.66,0.68,0.74 and 0.86mg/L respectively.The recoveries ranged from 93.5% to 105% and relative standard deviations（RSDs） were less than 4.3%（n=3）.This method was simple,rapid,effective and it could be used for the quality control of cosmetics.

关 键 词：微乳毛细管电泳 糖皮质激素 离子液体 Β-环糊精 化妆品 

分 类 号：O652.6[理学—分析化学]