紫外线通过诱导TNF-α胞内聚集间接调控VEGF分泌表达  

Intracellular accumulation of TNF-α is involved in VEGF induction upon ultraviolet B irradiation

作　　者：胡美茹[1] 董雯[1] 李译[1,2] 买三月[1] 李小光[1,2] 郭宁[1] 宋伦[1] 

机构地区：[1]军事医学科学院基础医学研究所病理生理学研究室,北京100850 [2]中国药科大学新药筛选中心,南京210009

出　　处：《军事医学》2012年第1期24-26,共3页Military Medical Sciences

基　　金：国家自然科学基金资助项目(30871277;30970594;31171342);北京市自然科学基金资助项目(5092022;5102035);国家重点基础研究发展规划资助项目(2011CB503803)

摘　　要：目的探讨紫外线(UVB)反应中TNF-α在介导VEGF诱导表达中的作用。方法采用TNF-α特异性shRNA转染小鼠成纤维细胞(MEFs)并建立稳定细胞株;RT-PCR方法验证TNF-αshRNA的有效性;荧光素酶报道分析系统检测vegf基因启动子活性;ELISA方法检测VEGF的分泌表达水平。结果本课题组构建的TNF-αshRNA能够有效抑制UVB刺激作用下TNF-α的诱导表达;同样条件下,UVB诱导vegf基因启动子活性上调趋势被TNF-αshRNA显著抑制;同时VEGF的胞外分泌表达水平明显下降。结论 UVB反应中TNF-α可间接介导VEGF的诱导表达。Objective To explore the role of TNF-α in mediating VEGF expression under UVB exposure.Methods The expression plasmid containing TNF-α short hairpin RNA（shRNA） was introduced into the mouse embryonic fibroblasts（MEFs） and then the stable transfectants were established.The efficiency of TNF-α shRNA in blocking TNF-α induction under UVB irradiation was confirmed by RT-PCR assay.The transcription of vegf under UVB irradiation was determined by the luciferase reporter assay.The secretion of VEGF induced by UVB was determined by ELISA.Results TNF-α shRNA efficiently blocked UVB-induced TNF-α transcription.Under the same condition,VEGF transcription and expression were significantly inhibited.Conclusion TNF-α induction is involved in VEGF expression in the cellular UVB response.

关 键 词：紫外线 TNF-Α VEGF 

分 类 号：R363[医药卫生—病理学]