乙酰肝素酶、MMP-9在膀胱移行细胞癌侵袭转移中的表达相关性及临床意义  

HPA and MMP-9 in the expression of invasion and metastasis of bladder transitional cell carcinoma and clinical significanc

作　　者：陈芳[1] 李丽[2] 

机构地区：[1]河南大学淮河医院肾内科,河南开封475001 [2]河南大学护理学院,河南开封475001

出　　处：《中国医药导报》2012年第1期22-24,共3页China Medical Herald

摘　　要：目的:检测乙酰肝素酶(heparanase,HPA)和基质金属蛋白酶-9(matrix metalloproteinase-9,MMP-9)在膀胱移行细胞癌、正常膀胱组织中的表达及相互关系,分析其与临床预后及浸润转移的关系。方法:应用免疫组化SP法检测92例膀胱移行细胞癌和10例正常膀胱组织中HPA、MMP-9的表达水平,分析两者的表达与临床病理指标的关系,建立Pearson等级相关分析。结果:HPA在膀胱移行细胞癌中的表达明显高于正常膀胱组织(77.2%,0;P<0.05),HPA阳性表达与周围组织浸润(χ2=11.918,P<0.05)、淋巴结转移(χ2=5.380,P<0.05)有关,与性别、年龄、组织学分级及1年生存率无关(P>0.05);膀胱移行细胞癌组织中MMP-9表达明显高于正常膀胱组织(70.7%,20.0%;P<0.05),且MMP-9阳性表达与周围组织浸润(χ2=4.329,P<0.05)、远处转移(χ2=10.301,P<0.05)有关,与性别、年龄、组织学分级及1年生存率无关(P>0.05)。HPA与MMP-9有较高的共同阳性表达率(P=0.018)。HPA和MMP-9表达呈正相关(r=0.729,P<0.05),其共同表达率与肿瘤对周围脏器浸润和淋巴结转移有相关性(P<0.05)。结论:HPA、MMP-9在膀胱移行细胞癌组织中的表达可能与膀胱移行细胞癌浸润、转移相关,可作为新的膀胱移行细胞癌标记物用于联合检测,具有重要的临床意义。Objective： To investigate the expression of heparanase and matrix metalloproteinase-9（MMP-9）,and their correlations with the metastasis and prognosis of bladder transitional cell carcinoma.Methods： Immunohistochemical staining（SP method） was used to measure HPA and MMP-9 expression in 92 cases of bladder transitional cell carcinoma and 10 cases of normal pancreas.Correlations of the expression of HPA and MMP-9 to clinicopathologic features were analyzed.Pearson regress ionmodel was used to analyze.Results： The expression of HPA in bladder transitional cell carcinoma was significantly higher than that in normal bladders（77.2%,0;P0.05）,the HPA protein intensive expression was associated with the infiltration of other tissue（χ2=11.918,P0.05） and lymph nodemetastasis（χ2=5.380,P0.05）,whereas there were no relation with gender,age,histological grade and 1-year postoperative survival（P0.05）;the expression of MMP-9 in bladder transitional cell carcinoma was significantly higher than that in normal bladders（70.0%,20.0%;P0.05）,the MMP-9 protein intensive expression was associated with the infiltration of other tissue（χ2=4.329,P0.05） and lymph node metastasis（χ2=10.301,P0.05）,whereas there were no relation with gender,age,tumor differentiation and 1-year postoperative survival（P0.05）.HPA and MMP-9 expression had a higher positive rate in bladder transitional cell carcinoma（P=0.018）.The positive expressions of HPA and MMP-9 was correlated in the bladder transitional cell carcinoma（r=0.729,P0.05）.Conclusion： HPA and MMP-9 may be associated with the infiltration and metastasis of the bladder transitional cell carcinoma.HPA and MMP-9 may be important features of cancer of bladder.A combined detection of them may benefit us in prediction of the progression of bladder transitional cell carcinoma.

关 键 词：乙酰肝素酶 基质金属蛋白酶-9 膀胱移行细胞癌 

分 类 号：R737.14[医药卫生—肿瘤]