Characterization of spontaneous breast tumor in tree shrews(Tupaia belangeri chinenesis)  

自发性树鼩乳腺肿瘤的特性(英文)

作　　者：夏厚军[1] 王春艳[1,2,3] 张海林[1] 何保丽[3] 角建林[3] 陈策实[1] 

机构地区：[1]中国科学院和云南省动物模型与人类疾病机理重点实验室中国科学院昆明动物研究所,云南昆明650223 [2]中国科学院研究生院,北京100039 [3]昆明医学院,云南昆明650031

出　　处：《Zoological Research》2012年第1期55-59,共5页动物学研究（英文）

基　　金：Yunnan Province High-Profile Talent Project (2010CI114);the Chinese Academy of Sciences,Basic Frontier Project (KSCX2-EW-J-23)

摘　　要：Breast cancer is a common malignant tumor.It is essential to develop suitable animal models for discovering novel preventive and therapeutic approaches.Tree shrews（Tupaia belangeri chinensis） have a closer evolutionary relationship with humans than do rodents,which have been widely used in laboratory research.Spontaneous breast tumors were identified in tree shrews in 1960s;however,no detailed studies about tree shrew breast tumors have been conducted to date.Here,we characterized a spontaneous breast tumor from tree shrews by Haematoxylin Eosin（HE） staining.This tumor was identified as a papillary tumor.Immunohistochemical staining（IHC） for progesterone receptor（PR）,Ki-67 and cleaved caspase-3 showed that tumor cells were positive for PR,highly proliferative,and less apoptotic compared to normal breast epithelial cells.Thus,the spontaneous tumor of tree shrew is very close to human papillary tumors in terms of morphology and pathology and we concluded that tree shrew may be a suitable animal model for breast cancer research.乳腺癌是严重危害女性健康的常见恶性肿瘤,建立合适的乳腺癌动物模型对于研究人类乳腺癌的生物学机制及发展新的防治方法至关重要。相对于常用的啮齿类动物,树鼩(Tupaia belangeri chinensis,tree shrew)因在进化层次上更接近于人类而可用于建立更适合的乳腺癌模型。该文详细了介绍一例树鼩自发性乳头状良性乳腺肿瘤。免疫组化结果显示该例肿瘤孕激素受体阳性且Ki-67阳性细胞比例显著增加;而活化的Caspase3阳性细胞比例较低;且肿瘤的形态和病理与人导管内乳头状肿瘤非常接近。提示利用树鼩建立乳腺肿瘤模型的可行性。

关 键 词：Spontaneous breast cancer Tree shrew IMMUNOHISTOCHEMISTRY 

分 类 号：R737.9[医药卫生—肿瘤]