Hedgehog信号通路在胃癌侵袭转移中的作用及分子机制  

Role of Hedgehog signaling pathway in invasion and metastasis of gastric cancer and relevant molecular mechanism

作　　者：郝亚琴[1] 欧阳小波[1] 代剑波[2] 王立[1] 

机构地区：[1]重庆医科大学附属第一医院消化内科,重庆400016 [2]重庆医科大学附属第二医院普外科,重庆400010

出　　处：《中国生物制品学杂志》2012年第2期171-175,共5页Chinese Journal of Biologicals

摘　　要：目的探讨Hedgehog(Hh)信号通路与上皮间质转换(Epithelial-mesenchymal transitions,EMT)在胃癌侵袭转移中的作用及分子机制。方法分别用环靶明、Snail siRNA、空质粒及环靶明+Snail siRNA的混合物处理人胃癌SGC-7901细胞,并设常规培养对照组,48 h后,采用RT-PCR法检测各组细胞中Gli1、Snail及E-cadherin基因mRNA的转录水平;Transwell小室试验检测细胞侵袭能力的变化;异质黏附试验检测各处理因素对细胞异质黏附能力的影响。结果与对照组相比,环靶明干扰组和联合干扰组细胞Gli1基因mRNA的转录水平明显降低(P<0.05),环靶明干扰组、Snail siRNA组及联合干扰组Snail基因mRNA的转录水平明显降低(P<0.05),而E-cadherin基因mRNA的转录水平明显升高(P<0.05);环靶明干扰组、Snail siRNA组及联合干扰组中细胞的侵袭能力及异质黏附能力明显低于对照组(P<0.05)。结论 Hh信号通路可能通过Gli1调控Snail而参与调控EMT的过程,从而在胃癌的侵袭转移中发挥重要作用。Objective To investigate the roles of Hedgehog （Hh） signaling pathway and epithelial-mesenchymal transition （EMT） in invasion and metastasis of gastric cancer as well as the relevant molecular mechanism. Methods Human gastric carcinoma SGC-7901 cells were treated with cyclopamine, Snail siRNA, empty plasmid and cyclopamine ＋ Snail siRNA for 48 h respectively, using those in routine culture as control. The cells in various groups were determined for transcription levels of Glil, Snail and E-cadherin mRNAs by RT-PCR, for invasion ability by Transwell test, and for effect on adhesion ability to foreign matter by adhesion test on Marigel glue. Results Compared with those in control group, the transcription level of Glil mRNA in cyclopamine and cyclopamine ＋ Snail siRNA groups decreased significantly （P 〈 0. 05）; however, the transcription levels of Snail mRNA in cyclopamine, Snail siRNA and cyclopamine ＋ Snail siRNA groups decreased significantly, while that of E-cadherin mRNA increased significantly （P 〈 0. 05）. Both the invasion abilities and adhesion abilities to foreign matters of cells in cyclopamine, Snail siRNA and cyclopamine ＋ Snail siRNA groups decreased significantly as compared with those in control group （P 〈 0. 05）. Conclusion Hh signaling pathway may be involved in regulation of Snail by Glil to low the expression of E-cadherin, which results in EMT, thus plays an important role in the invasion and metastasis of gastric cancer.

关 键 词：胃肿瘤 肿瘤侵袭 肿瘤转移 HEDGEHOG信号通路 上皮间质转换 

分 类 号：R735.2[医药卫生—肿瘤]