小干扰RNA干扰缺氧诱导因子-1α增强口腔鳞癌细胞株化疗敏感性  

Enhancement of Chemotherapeutic Drug Sensitivity on Oral Squamous Cell Carcinoma Cells by Silencing Hypoxia-Inducible Factor 1α Gene with Small Interfering RNA

作　　者：李德超[1] 王健平[1] 朱杨[1] 姚丛珊[1] 

机构地区：[1]佳木斯大学附属口腔医院颌面外科,黑龙江佳木斯154004

出　　处：《生物技术通讯》2012年第1期49-51,共3页Letters in Biotechnology

基　　金：教育部留学回国人员科研启动基金(教外司留[2007]24号);黑龙江省自然科学基金(D201025);黑龙江省人事厅留学回国人员科技项目择优资助基金(黑人发[2009]23号)

摘　　要：目的:探讨针对缺氧诱导因子-1α(HIF-1α)的小干扰RNA(siRNA)对口腔鳞癌细胞(OSCC)化疗敏感性的影响。方法:用Western印迹检测OSCC和针对HIF-1α基因的siRNA导入OSCC后的HIF-1α蛋白表达水平;用MTT法检测细胞对化疗敏感性的影响;用流式细胞术检测化疗诱导细胞凋亡的凋亡率。结果:HIF-1α在OSCC中高表达,HIF-1α-siRNA转染后HIF-1α表达水平明显下降,细胞对化疗敏感性明显提高,化疗诱导肿瘤细胞凋亡率明显增加。结论:针对HIF-1α基因的siRNA能明显降低HIF-1α的表达,增强化疗对OSCC的凋亡诱导作用,有效提高OSCC对化疗的敏感性。Objective： To investigate the feasibility of using small interfering RNA（siRNA） targeting hypoxia-inducible factor（HIF） 1α gene to enhance the drug sensitivity of oral squamous cell carcinoma cells（OSCC） to chemotherapeutic drugs.Methods： The expression of HIF-1α in OSCC and HIF-1α-siRNA transfected cells was examined by Western blot.Inhibition rate to chemotherapeutic drugs was examined by MTT assay.The apoptotic ratio was determined by flow cytometry analysis.Results： The expression of HIF-1α in HIF-1α-siRNA transfected OSCC were decreased and the cells were sensitive to chemotherapeutic drugs.The apoptotic ratio was significantly inereased in HIF-1α-siRNA transfected OSCC.Conclusion： Silencing HIF-1α gene with siRNA reduced the expression of HIF-1α,enhanced apoptosis and sensitivity of OSC cells to chemotherapeutic drugs.

关 键 词：缺氧诱导因子-1Α 小干扰RNA 口腔鳞癌细胞 化疗敏感性 

分 类 号：Q78[生物学—分子生物学] R730.5[医药卫生—肿瘤]