中国南海新α芋螺毒素Mr1.8的合成及二硫键测定  

Synthesis and Determination of Disulfide Bridges of a Novel Alpha-Conotoxin Mr1.8 from South China Sea

作　　者：胡洁[1] 刘娜[1] 李海鹰[1] 刘珠果[1] 戴秋云[1] 

机构地区：[1]军事医学科学院生物工程研究所,北京100071

出　　处：《生物技术通讯》2012年第1期102-104,共3页Letters in Biotechnology

基　　金：国家重点基础研究发展计划(2010CB529802);国家自然科学基金(9071028)

摘　　要：目的:采用固相方法合成新型α4/7芋螺毒素Mr1.8(PECCTHPACHVSNPELC-NH2),并测定其折叠后的二硫键配对方式。方法:采用Fmoc-固相法合成线性肽Mr1.8,通过空气氧化折叠获得含二硫键的折叠产物,利用两步折叠法测定其二硫键连接方式。结果:Mr1.8线性肽经折叠生成2种产物Ⅰ和Ⅱ,质谱和二硫键分析结果显示Mr1.8-Ⅱ为正确折叠产物,其二硫键框架为(Cys1-Cys3,Cys2-Cys4)。结论:Mr1.8是一种新的α4/7型芋螺毒素,其一种主要折叠产物的二硫键框架为(Cys1-Cys3,Cys2-Cys4)。Objective： To synthesize the novel α4/7 conotoxin Mr1.8（PECCTHPACHVSNPELC-NH2）,and determine its disulfide bridges.Methods： The linear Mr1.8 was synthesized using Fmoc solid-phase method,which was oxidized by air to give the product containing disulfide bridges.Then step-wise oxidation was employed to identify the disulfide-bridges framework.Results： Two main folded products were observed in one-step oxidative folding,and the mass spectrometry and disulfide-bridges analysis suggested that the main product folded with the disulfide bridges of ＂Cys1-Cys3,Cys2-Cys4＂.Conclusion： A novel α-conotoxin Mr1.8 with the sulfide bridges ＂Cys1-Cys3,Cys2-Cys4＂ was synthesized.

关 键 词：芋螺 芋螺毒素 合成 二硫键 烟碱型乙酰胆碱受体 

分 类 号：Q516[生物学—生物化学] Q502