丙烯酰胺对大鼠血脑脊液屏障通透性损伤效应的研究 丙烯酰胺对大鼠血脑脊液屏障通透性损伤效应的研究  

Effects of acrylamide on the permeability of blood cerebrospinal fluid barrier in rats

作　　者：姚雪[1] 曾凡旭[1] 姚林[1] 闫立城[1] 王苗苗[1] 王曼曼[1] 郝玉兰[1] 张艳淑[1] 

机构地区：[1]河北联合大学公共卫生学院毒理教研室,唐山063000

出　　处：《中华劳动卫生职业病杂志》2012年第2期81-84,共4页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：国家自然科学基金资助项目（30771823）

摘　　要：目的 探讨丙烯酰胺（ACR）对大鼠血脑脊液屏障通透性及脉络丛紧密连接蛋白ZO-1的影响,为阐明ACR神经损伤机制提供理论依据.方法 健康雄性SD大鼠32只随机分为对照组和ACR组,每组16只.ACR组按20 mg/kg体重腹腔注射丙烯酰胺溶液（1 ml/kg）,每周5次,连续4周；对照组注射等量的生理盐水.每周进行神经（感觉和运动）功能测试.染毒结束后测定脑脊液中伊文氏蓝（EB）和荧光素钠（NaFI）含量,实时荧光定量PCR（ real-time PCR）检测脉络丛上皮细胞紧密连接蛋白ZO-1的mRNA表达,激光共聚焦免疫荧光法观察脉络从紧密连接蛋白ZO-1的分布.结果 ACR组大鼠染毒第3周时,大鼠甩尾时间比对照组延长27.77％;染毒第4周时,甩尾时间延长53.71％；差异均有统计学意义（P＜0.05）.ACR组大鼠染毒第3周时,大鼠后肢展开距离增加明显,是对照组的131.76％；染毒第4周后肢展开距离是对照组的153.77％；差异均有统计学意义（P＜0.05）.ACR染毒组大鼠脑脊液中EB和NaFI含量均明显高于对照组,差异有统计学意义（P＜0.05）.ACR染毒4周后,大鼠脉络丛中ZO-1 mRNA表达为0.21 ±0.07,与对照组[（0.31±0.11）]比较,下降32.26％,差异有统计学意义（P＜0.05）.激光共聚焦免疫荧光检测结果显示ACR组染毒4周后大鼠脉络丛紧密连接蛋白ZO-1表达减少.结论 ACR可致染毒大鼠血脑脊液屏障通透性增高,脉络丛中紧密连接蛋白ZO-1表达下降,可能与ACR导致中枢神经损伤有关.Objective To explore the effects of acrylamide on the permeability of blood cerebrospinal fluid barrier （BCB） and tight junction protein ZO-1 of choroid plexus in rats and to provide a theoretical basis for explaining the mechanism of nerve injury induced by acrylamide.Methods Thirty two male Sprague-Dawley rats were randomly divided into ACR and control groups.ACR group was exposed to 20 mg/kg ACR daily for 5 days a week by intraperitoneal injection （i.p.） for 4 weeks.Control group was exposed to normal saline.The neurobehavioral tests （including sensatory and motor functions） were performed every week.At the end of exposure,Evan blue （EB） and Sodium fluorescein （NaFI） content in rat CSF were detected for determining the BCB permeability,Real-time PCR was used to measure the expression levels of ZO-1 mRNA in the epithelium cells of choroid plexus,and Laser Scanning Confocal Microscope （LSCM） was utilized to observe the distribution of ZO-1 protein.Results Neurobehavioral tests showed that the tail-flick latencies of ACR group were 27.77% and 53.71% as long as control group in the 3rd week and 4th week,respectively （P〈0.05）.The hind lamb splay distances of ACR group were 131.76% and 153.77% as long as control group in the 3rd week and 4th week,respectively （P〈0.05）.Evan blue （EB） and Sodium fluorescein （NaFI） content of ACR group were significantly higher than those of control group （P〈0.05）.In the 4th week,the expression level ofZO-1 mRNA in ACR group was 0.21±0.07,which was significantly lower than that （0.31±0.11） in control group （P〈0.05）.In the 4th week,the ZO-1 protein expression level of choroid plexus in ACR group was significantly lower than that in control group （P〈0.05）.Conclusion Acrylamide could increased the BCB permeability of rats,which may be involved in the central nervous injury induced by ACR.

关 键 词：丙烯酰胺 血脑屏障 脉络丛 

分 类 号：R743.310.2[医药卫生—神经病学与精神病学]