白细胞淤滞对糖尿病视网膜病变作用的研究  

Study of the effect of leukostasis in diabetic retinopathy

作　　者：邴寒[1] 唐杰[1] 安小玲[1] 赫红丹[1] 

机构地区：[1]黑龙江省医院南岗分院眼科,中国黑龙江省哈尔滨市150001

出　　处：《国际眼科杂志》2012年第2期218-220,共3页International Eye Science

摘　　要：目的:观察糖尿病小鼠视网膜血管内白细胞淤滞和细胞间黏附因子-1(ICAM-1)在视网膜的表达。方法:选取C57型小鼠45只,随机分为2组:糖尿病组22只、正常组23只。10wk后取视网膜,荧光显微镜计数小鼠全视网膜微血管内淤滞的白细胞数目及其含有ICAM-1表达的荧光小球数目,免疫蛋白印迹法检测视网膜血管内皮生长因子(VEGF)和ICAM-1的表达。结果:10wk后,糖尿病小鼠视网膜血管内白细胞数目及含有ICAM-1表达的荧光小球数目明显高于正常对照组(P<0.01),其视网膜内VEGF和ICAM-1明显增加,有统计学意义(P<0.05)。结论:视网膜血管内白细胞淤滞与糖尿病视网膜病变(DR)早期的视网膜毛细血管无灌注有关。AIM：To investigate the leukostasis and expression of ICAM-1 in 10 weeks diabetic mice retina.METHODS：Forty-five C57 mice（20-25g） were randomly divided into two groups.There were twenty-two mice in diabetic group and twenty-three mice in normal group.The leukostasis in diabetic retinopathy was counted and the expression of VEGF and ICAM-1 was studied by Western Blot.RESULTS：Ten weeks postoperatively,the number of leukocyte was significantly high in diabetic retinopathy（P0.01）.The expression of VEGF and ICAM-1 was significantly increased in diabetic group（P0.05）.CONCLUSION：The no perfusion of retinal blood capillary is related to leukostasis in early diabetic retinopathy.

关 键 词：视网膜 白细胞 糖尿病视网膜病变 细胞间黏附因子-1 

分 类 号：R774.1[医药卫生—眼科]