桑葚多糖提取方法的比较  

Comparative Study on the Methods for Extracting Polysaccharides from FRUCTUS MORI

作　　者：刘胜利[1] 刘晓露[1] 黄锁义[1] 黄礼德[1] 郭立强[1] 黄少花[1] 潘喜英[1] 

机构地区：[1]右江民族医学院,广西百色533000

出　　处：《安徽农业科学》2012年第5期2699-2700,2705,共3页Journal of Anhui Agricultural Sciences

基　　金：广西自然科学基金资助(2011GXNSFA018046);右江民族医学院2010-2012年度普高学生科研项目(右医科字〔2010〕9号);右江民族医学院民族医药协会资助项目(20100105)

摘　　要：[目的]比较不同提取方法对桑葚多糖提取的影响。[方法]以桑葚多糖得率为考察指标,比较酶提取、超声辅助提取及2种方法联合提取效果的优劣,并采用正交试验优化桑葚多糖的酶提取及超声提取条件。[结果]3种提取工艺中,酶-超声联合提取多糖得率最高,酶辅助提取次之,超声提取最低。[结论]酶-超声联合提取方法是一种高效的提取方法,可用于桑葚多糖的提取。[Objective] To compare the effect of different methods on the extraction of polysaccharides from FRUCTUS MORI.[Method] Taking the yield of polysaccharides as index,the advantage and disadvantage of 3 methods including enzymatic extraction,ultrasonic extraction and a combination of these two processes were compared.And,the extraction process of polysaccharides was optimized by orthogonal test.[Result] The highest yield of polysaccharides was obtained from FRUCTUS MORI by using ultrasonic combined with enzyme,followed by enzyme-assisted extraction and ultrasonic extraction individually.[Conclusion] Ultrasonic combined with enzyme is a high-efficient method to extract polysaccharides from FRUCTUS MORI.

关 键 词：桑葚多糖 纤维素酶 超声波 

分 类 号：O636.1[理学—高分子化学]