高效液相色谱法同时测定食品中7种非食用色素  

Determination of Seven Inedible Pigments in Food Using High Performance Liquid Chromatography

作　　者：范文锐[1] 吴青[1] 劳扬[1] 卢玉珊[1] 

机构地区：[1]华南农业大学食品学院,广东省食品质量安全重点实验室,广州510642

出　　处：《分析化学》2012年第2期292-297,共6页Chinese Journal of Analytical Chemistry

基　　金：广东省大学生创新实验资助项目(No.1056410025)

摘　　要：建立了高效液相色谱法同时测定食品中7种非食用色素(碱性嫩黄O、碱性橙、酸性橙Ⅱ、酸性金黄、玫瑰红B、对位红、苏丹红Ⅰ)的方法。依次采用乙腈、甲醇和碱性甲醇提取豆制品和肉制品;采用乙腈和70%乙腈依次提取调味品。采用SunFireTMC18色谱柱(250 mm×4.6 mm,5μm),以甲醇-50 mmol/L乙酸铵水溶液(H3PO4调至pH 4.5)为流动相,梯度洗脱,流速1 mL/min,测定波长为450和520 nm。7种非食用色素的在各自相应浓度范围内线性相关系数均大于0.998;检出限(LOD)在0.01～0.1 mg/L之间;定量限(LOQ)在0.18～1.2 mg/kg之间。平均回收率均大于80%;相对标准偏差(RSD,n=3)在2.0%～5.7%之间。A method for the simultaneous determination of seven inedible pigments（Auramine O,Basic orange,Acid orangeⅡ,Acid golden yellow G,Rhodamine B,Para Red,SudanⅠ） in food was developed using high performance liquid chromatograph with ultraviolet-visible detector.Acetonitrile,methanol and basic methanol were used to extract the inedible pigments from bean and meat products.Acetonitrile and 70% acetonitrile-water were used to extract the inedible pigments from condiments.Using SunFireTM C18（250 mm×4.6 mm,5 μm）,methanol-50 mmol/L ammonium acetate（adjust pH to 4.5 with phosphoric acid） as the mobile phase with a gradient elution at the rate of 1 mL/min and detected at the wavelength of 450 and 520 nm.The linear ranges of seven inedible pigments were well with correlation coefficient above 0.998.The limit of detection（LOD） of seven inedible pigments was 0.01 mg/L to 0.1 mg/L and the limit of quantification（LOQ） was 0.18 mg/kg to 1.25 mg/kg.The average recoveries of seven inedible pigments were above 80%.The RSD（n=3） was from 2.0% to 5.7%.

关 键 词：高效液相色谱 非食用色素 碱性嫩黄O 碱性橙 酸性橙Ⅱ 酸性金黄 玫瑰红B 对位红 苏丹红Ⅰ 

分 类 号：R155.5[医药卫生—营养与食品卫生学]