离子液体双水相溶剂浮选法分离/富集桑黄黄酮类成分  

Separation/Richment Total Flavonoids in Phellinus Igniarius by Ionic Liquid/Salt Aqueous Two-phase Flotation

作　　者：戈延茹[1] 潘如[1] 傅海珍[1] 吴云[1] 付文艳[1] 闫永胜[2] 戚雪勇[1] 

机构地区：[1]江苏大学药学院,镇江212013 [2]江苏大学化学化工学院,镇江212013

出　　处：《分析化学》2012年第2期317-320,共4页Chinese Journal of Analytical Chemistry

基　　金：江苏大学高级人才引进专项资助项目(No.1283000138);江苏省普通高校研究生科研新计划(No.CXLX11_0600)资助

摘　　要：将亲水性离子液体氯化-1-丁基-3-甲基咪唑([C4mim]Cl)和K2HPO4形成的双水相体系与溶剂浮选结合,建立了分离/富集桑黄中总黄酮类成分的方法。考察了分相盐的种类和用量、样品量、溶液pH值、浮选时间和氮气流速对浮选效果的影响,并与双水相萃取进行比较。当浮选分相盐K2HPO4的质量浓度为50%、溶液pH=9.53、离子液体的用量为3 mL、浮选时间为50 min、氮气流速为30 mL/min时,浮选效率最佳,达到85.31%,富集倍数为8.59。离子液体双水相溶剂浮选法浮选效率高,富集倍数大,为中草药有效成分分离/富集提供了新方法。Ionic liquid/salt aqueous two-phase flotation coupled with UV spectrophotometry was developed for the separation/enrichment and analysis of total flavonoids in Phellinus igniarius（Sanghuang）.The method was combined with ionic liquid aqueous two-phase system based on ionic liquid（1-butyl-3-methylimidazolium chloride,Cl） and inorganic salt（K2HPO4） with solvent sublation.The effect on the flotation efficiency was studied.The best conditions were as follows： the concentration of K2HPO4 was 50%;pH of solution was 9.53;the amount of Cl was 3 mL;the flotation time was 50 min;the nitrogen flow rate was 30 mL/min.The flotation efficiency was 85.31%,the enrichment factor was 8.59.These values are higher and larger than those of two-phase extraction.The method is suitable for the separation and enrichment of the active ingredient of Chinese medicine.

关 键 词：溶剂浮选 紫外分光光度法 离子液体 双水相 桑黄 总黄酮 

分 类 号：R284.1[医药卫生—中药学]