二至丸保肝活性成分群对体外肝细胞损伤的保护作用  

Protective Effect of Active Ingredients Group in Liver Protection from Erzhi Pill on Hepatocyte Injury Induced by CCl_4 or H_2O_2

作　　者：蔡秀江[1] 闫冰[1,2] 姚卫峰[1] 张丽[1] 黄美艳[1] 丁安伟[1] 

机构地区：[1]南京中医药大学江苏省方剂高技术研究重点实验室,江苏南京210046 [2]南京军区南京总医院制剂科,江苏南京210002

出　　处：《辽宁中医杂志》2012年第2期212-215,共4页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家自然科学青年基金项目(81001599);教育部博士点新教师基金(20103237120010);江苏省中医药管理局研究项目(LB09031)

摘　　要：目的:研究二至丸保肝活性成分群(70%乙醇部位)(active ingredients group in liver protection from Erzhi Pill,AIEP)对体外肝细胞损伤的保护作用及其机理。方法:培养L-O2型肝细胞,采用CCl4和H2O2体外诱导肝细胞损伤,检测培养上清液中天门冬氨酸转换酶(AST)和丙氨酸氨基转换酶(ALT)水平,测定上清液中丙二醛(MDA)的含量和过氧化物歧化酶(SOD)活力及谷胱甘肽过氧化物酶(GSH-Px)活性,MTT法检测细胞存活和增殖活性。结果:(1)AIEP(0.32～40μg.mL-1)剂量组可明显降低由CCl4所致肝细胞培养上清液中AST和ALT水平及MDA含量的升高,还可显著提高由CCl4所致肝细胞存活率和SOD活力及GSH-Px活性的降低;(2)AIEP(0.32～40μg.mL-1)剂量组可使H2O2升高的肝细胞培养上清液中AST和ALT水平及MDA含量明显降低或恢复,还可显著提高CCl4降低的肝细胞存活率和SOD活力及GSH-Px活性。结论:提示AIEP对体外肝细胞损伤有较强的保护作用,该作用可能与其抗氧化作用有关。Objective ：To determine active ingredients group in liver protection from Erzhi Pill （AIEP） on hepatocyte injury induced by CCl4 or H2 O2, and to reveal the mechanism. Methods ： L - O2 hepatocyte ceus, were incubated, and then injured by CCl4 or H2 O2. The levels of AST, ALT, MDA, SOD and GSH - Px in cultural supernatants were detected. Cell viability was assayed by MTT reduction method. Results ：The CCl4 - induced elevation of MDA content in hepatocytes increased AST and ALT levels in the supernatant of hepatocytes, and loss of SOD and GSH - Px activities were remarkably attenuated by AIEP （0.32 - 40 μg ·mL^- 1 ）. The H2 O2 -induced elevation of ALT and AST levels in the supernatant of hepatocytes increased MDA content of hepatocytes, and loss of SOD and GSH -Px activities were significantly attenuated by i AIEP （0. 32 - 40 μg ·mL^- 1）. Conclusion：AIEP directly protects hepatocytes from in vitro injury induced by CCl4 or H2 O2. This might be associated with its anti - oxidative activity.

关 键 词：二至丸 保肝 L-O2型肝细胞 抗氧化作用 

分 类 号：R222.19[医药卫生—中医基础理论]