宝藿苷-Ⅰ联合As_2O_3对食管癌Eca-109细胞增殖的影响  

Effect of baohuoside-Ⅰ combined with As_2O_3 on proliferation of human esophageal carcinoma cell Eca-109

作　　者：苏镇军 郭莉莉[2] 刘晓霞[2] 郝玉栓[2] 孙立尧[1] 

机构地区：[1]邯郸钢铁集团有限责任公司职工医院,河北邯郸056001 [2]河北工程大学医学院,河北邯郸056029

出　　处：《现代中西医结合杂志》2012年第6期593-595,共3页Modern Journal of Integrated Traditional Chinese and Western Medicine

摘　　要：目的研究宝藿苷-Ⅰ与As2O3联合应用对食管癌细胞Eca-109增殖的影响。方法取对数生长期Eca-109接种于96孔培养板,宝藿苷-Ⅰ组予以25 mg/L宝霍苷As2O3组予0.18 mg/L As2O3,联合用药组予12.5 mg/L宝藿苷-Ⅰ+0.09 mg/L As2O3,对照组予0.1%DMSO。采用MTT法分析对Eca-109细胞增殖的抑制作用,DNA琼脂糖凝胶电泳分析细胞凋亡,流式细胞术分析细胞周期,Western Blot技术检测C-myc基因蛋白的表达,每组均重复3次。结果 3个用药组均可明显抑制Eca-109细胞的增殖(均P<0.05),但联合用药组抑制作用显著优于单独用药组(P<0.05);3个用药组均可使细胞凋亡,使细胞周期阻滞在G2/M期,使C-myc表达下调,与对照组相比均有显著性差异(P均<0.05),但联合用药组较单药组作用更显著(P<0.05)。结论各自半量的宝藿苷-Ⅰ与As2O3联合应用对Eca-109细胞的增殖抑制有显著的增强作用,明显优于各自的单味应用效果。Objective It is to observe the influence of of baohuoside- I combined with As203 on proliferation of human esophageal carcinoma cell Eca - 109. Methods The cells were divided into 4 groups： 25 mg/L baohuoside - I group （25 mg/ L） , As2O3 group （0.18 mg/L） , combination group （ 12.5 mg/L baohuoside - I and 0. 09 mg/L As203 ） , and control group （0.1% DMSO） , the inhibitory effect on proliferation of Eca - 109 ceils was analyzed by MTT, the apoptosis of Eca - 109 cells was measured with DNA agarose gel electrophoresis, the cell cycle was measured by flow cytometry, the expression of C- myc protein of Eca - 109 cells was measured with western blot, each group were repeated 3 times. Results The proliferation of Eca - 109 cells were inhibited in three drug groups （all P 〈0.05 ） , the inhibitory effect of combination group was superior to alone group （P 〈 0. 05） ; cell apoptosis of Eca- 109 cells was induced significantly in three groups, the cell rate of the G2/ M phase was increased significantly, expression of C - myc protein was decreased significantly than control group （ all P 〈 0. 05 ）, and the combination group showed more significant effect than alone group （ P 〈0.05 ）. Conclusion Half dose of Baohuoside- I and As2O3 in combination shows a synergistic enhancement on the inhibiting proliferation, and superior to alone group.

关 键 词：宝藿苷-Ⅰ AS2O3 细胞凋亡 C-MYC 食管癌 联合用药 

分 类 号：R735.1[医药卫生—肿瘤]