乳癖霜外用对乳腺增生模型大鼠乳腺组织病理形态学和AP-2α、C-erbB-2表达的影响  

Effect of Rupi cream on histomorphology and AP-2α and C-erbB-2 expressions in rats with hyperplasia of mammary gland

作　　者：马民[1] 李德辉[1] 张桂娟[2] 郭慧[1] 廖锐[1] 谭碧珠[1] 曾晓婷[1] 林逸民 黄斯[1] 

机构地区：[1]暨南大学医学院中医系,广东广州510632 [2]暨南大学附属第一医院

出　　处：《中国老年学杂志》2012年第4期738-742,共5页Chinese Journal of Gerontology

基　　金：国家自然科学基金资助项目(No.81173265);广东省自然科学基金项目(No.10151063201000065);广东省科技攻关项目(No.2009B030801238;2006B35602009);广州市科技支撑计划立项课题(2009Z1-E091);广东省中医药管理局项目(No.1060114;2008092;20111175);中央高校基本科研业务费专项资金资助;广东省大学生创新实验项目(No.1055910014);暨南大学2010年国家大学生创新性试验计划项目(No.101055916);暨南大学211工程建设项目;优秀本科推荐研究生科研创新培育计划项目

摘　　要：目的研究乳癖霜外用对乳腺增生模型大鼠乳腺组织病理形态学和AP-2α、C-erbB-2蛋白表达水平的影响。方法 36只大鼠随机分为正常对照组、疾病模型组、三苯氧胺软膏组、乳癖霜小、中、大剂量组。通过雌、孕激素联合制造乳腺增生病大鼠模型,造模的同时给予不同的药物进行干预。30 d后观察各组大鼠乳腺组织病理学和脏器系数的改变,放射免疫法检测各组大鼠血清性激素的水平,Western印迹法测定各组大鼠乳腺组织AP-2α蛋白的表达水平,免疫组化测定各组大鼠乳腺组织C-erbB-2蛋白的表达水平。结果乳癖霜外用能降低乳腺增生模型大鼠的乳头直径和高度,改善模型大鼠乳腺组织病理形态学改变,调节血清性激素水平,降低模型大鼠乳腺组织中转录因子AP-2α、癌基因C-erbB-2蛋白的表达水平,与模型组比较有统计学差异(P<0.01或P<0.05)。结论乳癖霜外用对乳腺增生模型大鼠有显著的疗效,其作用机制可能为调节血清性激素水平及调节大鼠乳腺组织转录因子AP-2α、原癌基因C-erbB-2蛋白的表达水平。Objective To observe the influence of external application of Rupi cream on histomorphology and AP-2α and C-erbB-2 expressions in rats with hyperplasia of mammary gland.Methods 36 virginal female SD rats were randomly allocated into normal control,disease model,tamoxifen ointment,high and middle and low dosage of Rupi cream groups.Rat models of experimental mammary hyperplasia were established by intramuscular injection of estrogen and progestin.After 30 d,the pathological of breast tissue,organ coefficient,sex hormones and the expressions of AP-2α and C-erbB-2 protein were observed.Results Compare with those of model group,Rupi cream reduced the nipple diameter and height,improved mammary tissue pathological,regulated serum sex hormone levels,decreased AP-2α and C-erbB-2 protein expressions of model rats（P〈0.01,P〈0.05）.Conclusions Rupi cream has significant effect on rats with mammary gland hyperplasia,its mechanism might be regulated the levels of serum sex hormone and expressions of AP-2α and C-erbB-2 protein.

关 键 词：乳癖霜 乳腺增生病 性激素 AP-2Α C-erbB-2 

分 类 号：R-33[医药卫生]