脂质体介导人NIS基因转染肺癌A549细胞及其蛋白表达  

Transfection of Human Sodium/Iodide Symporter(NIS) Gene with Liposome and the Expression of NIS Protein in A549 Cell

作　　者：张宏飞[1] 严煜[2] 张裕东[2] 龚德军[3] 

机构地区：[1]南通市第三人民医院,江苏南通226000 [2]南通大学附属医院,江苏南通226000 [3]第二军医大学附属长海医院胸心外科实验室,上海200433

出　　处：《临床肺科杂志》2012年第3期500-503,共4页Journal of Clinical Pulmonary Medicine

摘　　要：目的研究人钠/碘同向转运体(NIS)基因转染肺癌细胞及其蛋白表达。方法鉴定质粒pcDAN3-hNIS中的插入基因NIS基因。培养的肺癌A549分为两组:实验组(转染pcDAN3-hNIS),对照组(转染pcDAN3)。脂质体介导NIS基因转染肺癌细胞,采用Western Blot免疫印迹法和免疫组化法检测肺癌细胞中NIS蛋白的表达。结果实验组的肺癌细胞有NIS蛋白的表达,而对照组无表达,两组比较差异有显著性(P=0.000)。结论转染人NIS基因的肺癌细胞可表达NIS蛋白,为探索放射性碘治疗肺癌的研究提供理论依据。Objective To research the expression of human Sodium/Iodide symporter（hNIS） gene transfected to lung carcinoma A549 cells. Methods The hNIS gene was identified. Lung carcinoma cells cultured in vitro were transfected respectively by plasmid pcD- NA3-hNIS and pcDNA3 mediated by liposome vector. Western Blot and immunohistochemistry assay methods were used to detect the ex- pression products of NIS gene. Results The result of Western Blot and immunohistochemistry assay showed that there was positive expres- sion of NIS protein in experimental group. On the contrary, the expression of NIS protein was negative in control group. There was signifi- cant statistical difference between two groups （ P = 0. 000 ）. Conclusion Transfected lung carcinoma ceils could effectively express NIS protein in vitro. This will provide further the academic evidence in radioiodine therapy for lung cancer.

关 键 词：人钠/碘同向转运体 肺癌细胞 基因转染 脂质体 放射性碘治疗 

分 类 号：R734.2[医药卫生—肿瘤]