RP-HPLC法同时测定双丹口服液中没食子酸、丹参素和原儿茶醛的含量  

Simultaneous determination of gallic acid,danshensu and protocatechuic aldehyde in Shuangdan Oral Liquid by RP-HPLC

作　　者：李骅[1] 谢艳华[1] 张邦乐[2] 王剑波[1] 杨倩[1] 曹蔚[1] 王四旺[1] 

机构地区：[1]第四军医大学药学系药物研究所,陕西西安710032 [2]第四军医大学药学系药剂学教研室,陕西西安710032

出　　处：《中国医药导报》2012年第5期117-118,122,共3页China Medical Herald

摘　　要：目的建立同时测定双丹口服液中3种活性成分没食子酸、丹参素和原儿茶醛含量的分析方法。方法采用高效液相色谱法,色谱柱为Sino Chrom ODS-BP C18(250 mm×4.6 mm,5μm)柱,流动相为甲醇-3%冰醋酸水溶液(8∶92),流速为1.0 mL/min,柱温为30℃,检测波长为280 nm。结果没食子酸、丹参素和原儿茶醛分别在5.0～100.0μg/mL(r=0.999 8)、7.5～150.0μg/mL(r=0.999 9)、5.0～100.0μg/mL(r=0.999 8)范围内具有良好的线性关系,加样回收率(n=6)依次为99.2%、99.8%、100.3%。结论本方法操作简便,结果准确,重复性好,适用于双丹口服液的质量控制。Objective To establish a HPLC method for the simultaneous determination of gallic acid,danshensu and protocatechuic aldehyde in Shuangdan Oral Liquid.Methods HPLC was performed on Sino Chrom ODS-BP C18（250 mm × 4.6 mm,5 μm） column and the mobile phase was consisted of methanol-3% glacial acetic acid aqueous solution（8∶92）.The flow rate was 1.0 mL/min,the temperature of column was 30℃,and the wave length of detection was 280 nm.Results The linear relationship were good for gallic acid,danshensu and protocatechuic aldehyde within the range of 5.0-100.0 μg/mL（r=0.999 8）,7.5-150.0 μg/mL（r=0.999 9） and 5.0-100.0 μg/mL（r=0.999 8）,with the recoveries（n=6） of 99.2%,99.8% and 100.3%,respectively.Conclusion The established HPLC method is simple and relible with good reproducibility,which is suitable for the analysis and quality control of gallic acid,danshensu and protocatechuic aldehyde in Shuangdan Oral Liquid.

关 键 词：双丹口服液 没食子酸 丹参素 原儿茶醛 反相高效液相色谱法 含量测定 

分 类 号：R927.2[医药卫生—药学]