健康中国恒河猴各淋巴组织中T淋巴细胞表型及分布  

Phenotype and Distribution of the Lymphocyte in a Variety of Lymphoid Tissue in Healthy Chinese-origin Rhesus Macaques

作　　者：熊竞[1] 刘克剑[1] 吴芳新[1] 丛喆[1] 王卫[1] 陈霆[1] 魏强[1] 

机构地区：[1]中国医学科学院医学实验动物研究所,卫生部人类疾病比较医学重点实验室,国家中医药管理局人类疾病动物模型三级实验室,北京100021

出　　处：《中国畜牧兽医》2012年第2期86-91,共6页China Animal Husbandry & Veterinary Medicine

基　　金：“十二·五”国家科技重大专项(2012ZX10004-501,2012ZX10001007-008);协和青年基金资助项目

摘　　要：试验旨在明确T淋巴细胞在中国恒河猴各组织中的表型与分布,为疾病模型研究提供基础数据。取外周血、腹股沟淋巴结、肠系膜淋巴结及肠道组织,从中分离出淋巴细胞。使用流式细胞术检测分析各种表型的淋巴细胞在组织间的分布。结果表明,淋巴结中CD4+/CD8+T细胞比值高于外周血,肠道固有层中最低,三者差异显著。记忆性T细胞在外周血和肠道固有层T细胞中比重较大,而淋巴结中主要为幼稚T细胞。CD4+T细胞中中心记忆T细胞Tcm为主要亚群,而CD8+T细胞主要为效应记忆细胞Tem。外周血与肠道固有层中增殖T细胞比例相当,而淋巴结中T细胞增殖水平相对较低。各组织中CXCR4受体表达量普遍高于CCR5受体,其中肠道固有层CCR5受体表达水平最高。值得注意的是,有一小群表型CD3+CD4+CD8low的细胞仅在肠道固有层中存在,经分析其功能活性应高于肠道CD4单阳性T细胞。因此,测定了健康中国恒河猴各表型T淋巴细胞在多种淋巴组织中的基础数值,为相关模型研究奠定基础。To quantitate and phenotype the T lymphocytes in a variety of lymphoid tissue in healthy Chinese-origin rhesus macaques.Lymphocyte composition of peripheral blood（PBL）,inguinal lymph nodes（ILN）,mesenteric lymph nodes（MLN）,intestinal lamina propria（LP） from healthy rhesus monkeys was characterized in detail by flow cytometry.The results showed that CD4＋ /CD8＋ ratio in LN showed higher than that in the PBL and lowest in LP,there were significant differences among them.In the LN,most CD4＋ T cells were naive T cells while in the PBL and LP most CD4＋ T cells were memory T cells.In all lymphoid tissues,memory markers were Tcm from the vast majority of CD4＋ T cells,but most of the CD8＋ T cells expressed a memory phenotype of Tem.The population of proliferating CD4＋ and CD8＋ T cell was similar from both PBL and LP,and higher than that from LN.The chemokine receptors CXCR4 were expressed higher than CCR5 in all lymphoid tissues.28% of CD4＋ T cells in LP expressing the CCR5 that was the highest among all tissues.It was notable that there is small population of CD3＋ CD4＋ CD8low cells exist only in LP.The results provides a basic reference values for further research related to immunity or AIDS model by using macaques.

关 键 词：中国恒河猴 肠道固有层 多色流氏细胞术 记忆T细胞 KI67 CCR5 CXCR4 

分 类 号：Q813[生物学—生物工程]