野生乌苏里貉18S rDNA全序列的克隆测序及系统进化树分析  

Cloning,Sequencing and Phylogenetic Analysis 18S rDNA Gene in Wide Nyctereutes Procyonoides Matschie

作　　者：刘诚刚[1] 杜智恒[1] 白秀娟[1] 

机构地区：[1]东北农业大学动物科学技术学院,黑龙江哈尔滨150030

出　　处：《中国畜牧兽医》2012年第2期117-120,共4页China Animal Husbandry & Veterinary Medicine

基　　金：黑龙江省教育厅2010年度科学技术研究面上项目计划(11551034)

摘　　要：试验采用聚合酶链式反应方法,从野生乌苏里貉耳部肉中提取DNA,扩增出18SrDNA的全序列,并对其进行克隆测序,获得长度为1831bp的序列,并将序列提交到GenBank上,经BLAST分析结果表明,测序得到的乌苏里貉18SrDNA与其他物种同源性均在90%以上,说明18SrDNA基因具有较高的保守性,可以作为分类学当中系统进化分析的重要参考依据。In this study,18S rDNA gene was extracted from wild Nyctereutes procyonoides matschie＇s meat of ear,and amplified by polymerase chain reaction（PCR） method,then cloned and sequenced.A lengh of 1831 bp was cloned,the sequences had been sent to GenBank and registered,comparison of the sequence with that of other animals 18S rDNA gene was carried out through BLAST.The results showed that the homologies of the nucleotide sequence were above 90% with Nyctereutes procyonoides matschie,and 18S rDNA gene was conserved in evolution,which could be used as phylogenetic analysis of the important reference.

关 键 词：野生乌苏里貉 克隆测序 18SrDNA 系统进化树 

分 类 号：Q813[生物学—生物工程]