HPLC-ELSD法同时测定连翘叶中齐墩果酸和熊果酸  

Simultaneous Determination of Oleanolic Acid and Ursolic Acid in the Leaves of Forsythia suspensa by HPLC-ELSD

作　　者：赵韶华[1] 刘敏彦[1] 王玉峰[1] 许红辉[1] 张永锋[1] 王猛[1] 

机构地区：[1]石家庄以岭药业股份有限公司,河北石家庄050035

出　　处：《食品科学》2012年第2期224-226,共3页Food Science

基　　金：国家科技重大专项(2009ZX09313-003)

摘　　要：目的:建立高效液相色谱-蒸发光散射检测法(high performance liquid chromatography-evaporative lightscattering detector,HPLC-ELSD)法同时测定连翘叶中齐墩果酸和熊果酸。方法:色谱柱:Waters Symmetry C18(4.6mm×250mm,5μm);柱温25℃,流动相:甲醇-0.4%冰醋酸溶液(93:7,V/V),流速0.4mL/min;蒸发光散射检测器检测条件:漂移管温度80℃,气体压力25psi。结果:齐墩果酸在0.107～2.136μg范围内线性关系良好(r=0.9991),熊果酸在0.179～3.584μg范围内线性关系良好(r=0.9993);齐墩果酸和熊果酸的回收率分别为98.67%和9 8.5 6%。结论:此方法简便、准确,重现性良好,为评价连翘叶的质量提供可靠的分析方法。Objective： To establish an high performance liquid chromatography-evaporative light scattering detector（HPLCELSD） method for the simultaneous determination of oleanolic acid and ursolic acid in the leaves of Forsythia suspensa.Methods： The chromatographic separation was carried out on a Waters Symmetry C18column（4.6 mm× 250 mm,5μm） with a mobile phase composed of methanol and 0.4% acetic acid（93：7,V/V） at a flow rate of 0.4 mL/min.An evaporative light scattering detector was used to detect oleanolic acid and ursolic acid with a drift tube temperature of 80 ℃ and a gas pressure of 25 psi.Results： Both the developed calibration curves of oleanolic acid and ursolic acid revealed a good linear relationship over the ranges of 0.107-2.136 μg（r =0.9991） and 0.179-3.584μg（r= 0.9993）,respectively.Therecovery ratesof oleanolicacid and ursolic acid were 98.67% and 98.56%,respectively.Conclusion： A simple,accurate and credible HPLC-ELSD method has been developed,which is suitable for the quality contro1 of the leaves of Forsythia suspensa.

关 键 词：连翘叶 高效液相色谱-蒸发光散射检测法(HPLC-ELSD) 齐墩果酸 熊果酸 

分 类 号：R282.71[医药卫生—中药学]