高压氧对神经病理痛大鼠NOS系统的影响  

EFFECTS OF HYPERBARIC OXYGEN ON NOS SYSTEM IN NEUROPATHIC PAIN RATS

作　　者：韩光[1] 李璐[1] 孟凌新[1] 崔健君[2] 

机构地区：[1]中国医科大学附属盛京医院麻醉科,沈阳110004 [2]中国医科大学附属盛京医院疼痛科,沈阳110004

出　　处：《中国疼痛医学杂志》2012年第2期104-107,共4页Chinese Journal of Pain Medicine

基　　金：沈阳科学技术计划项目(合同编码:沈科技合(10140)号);计划项目编码:F10-205-1-41

摘　　要：目的:观察高压氧对神经病理痛大鼠一氧化氮合酶(nitric oxygen synthase,NOS)系统的影响。方法:40只雄性SD大鼠随机分成五组:即空白对照组(C组);假手术组(S组);坐骨神经慢性缩窄手术组(CCI组);高压氧预处理组(Pre-H组)与高压氧干预组(Post-H组),术后28 d取材观察其脊髓的免疫组化指标,对其NOS各亚型表达进行比较。结果:CCI术后nNOS,iNOS表达明显增加,Pre-H组和Post-H组的nNOS及iNOS含量较CCI组明显降低。eNOS含量在整个过程中变化不大。结论:高压氧可能通过抑制NOS系统表达产生对CCI大鼠疼痛的抑制作用。Objective：To evaluate the effcts of hyperbaric oxygen（HBO） on nitric oxygen synthase（NOS） system in neuropathic pain in chronic constriction injury（CCI） rats.Methods： Forty male SD rats were randomly divided into five groups： control group（C group）,sham group（S group）,chronic constriction injury group（CCI group）,pre-hyperbaric oxygen group（Pre-H group）,post-hyperbaric oxygen group（Post-H group）.The changes of nNOS,iNOS,eNOS in spinal cord on the day 28 after surgery were assessed.Results： The expression of nNOS and iNOS in CCI group,Pre-H group and Post-H group was significantly higher than that of S group;the expression of nNOS and iNOS in Pre-H group and Post-H groups was significantly lower than that in CCI group.The expression of eNOS didn’t change during the observation period.Conclusion： HBO treatment may affect neuropathic pain through the inhibition of NOS system in CCI rats.

关 键 词：高压氧 神经病理性疼痛 NOS 

分 类 号：R741[医药卫生—神经病学与精神病学]