用sp.2262菌制备D-对羟基苯甘氨酸反应动力学  

KINETICS OF ENZYMOLYSISES FOR PRODUCTION OF D-p-HYDROXYPHENYLGLYCINE BY sp.2262 CELL

作　　者：张衍坤[1] 张铭俊[1] 李京华[1] 李曙光[1] 虞星炬[1] 

机构地区：[1]中国科学院大连化学物理研究所,大连116012

出　　处：《化工学报》2000年第1期57-62,共6页CIESC Journal

基　　金：国家科委"九.五"攻关项目!(No 96 c0 2 0 2 0 1 )

摘　　要：研究了以DL -对羟基苯海因为原料 ,用sp 2 2 62恶臭假单胞菌 (Pseudomonasputida)直接酶催化生产D -对羟基苯甘氨酸的反应动力学 .考察了DL -对羟基苯海因溶解过程和海因酶、氨甲酰水解酶酶解反应的动力学特性 .建立了全过程的动力学模型并进行了计算 ,求解了过程的参数值 ,讨论了 pH值对各参数的影响 ,确定了反应的优化操作条件 .Kinetics of enzymolysises for the production of D p hydro xyphenylglycine from DL p hydro xyphenylhydantion by Pseudomonas Putida cells containing both hydroxypyrimidinehydrase and N carbamoyl D aminoacid amidohydrolase were studied. Dissolution process of DL p hydroxyphenylhydantoin and characteristics of both enzymes were investigated. The results showed that enzymolysis catalyzed by hydro xypyrimidinehydrase accorded approximately with first order kinetics, while that catalyzed by N carbamoyl D aminoacid amidohydrolase could be described by modified Michaelis Menton equation. It was found that enzymolysis catalyzed by N carbamoyl D aminoacid amidohydrolase was inhibited by the high concentration substrate and the byproduct NH 3. The kinetic model of whole process was developed. The values of model parameters were given. The influence of pH on model parameters was discussed and optimum operation conditions were established .

关 键 词：对羟基苯甘氨酸 酶催化 反应动力学 假单胞菌 

分 类 号：TQ245.4[化学工程—有机化工] O643.1[理学—物理化学]