非酒精性单纯性脂肪肝患者外周血CD4＋CD25＋调节性T细胞数量变化及临床意义  

The changes and clinical significance of the frequency of CD4＋ CD25＋ regulatory T cells in the peripheral blood of patients with nonalcoholic fatty liver

作　　者：程瑗 陈永平[1] 金晓芝[1] 戴志娟[1] 叶超[1] 章圣辉[3] 

机构地区：[1]温州医学院附属第一医院感染内科,325000 [2]湖南省宁乡县人民医院感染科,410600 [3]温州医学院附属第一医院内科实验室,325000

出　　处：《中华传染病杂志》2012年第1期43-47,共5页Chinese Journal of Infectious Diseases

摘　　要：目的探索非酒精性单纯性脂肪肝（NAFL）患者外周血CD4＋CD25＋调节性T细胞（Treg）数量变化与临床意义。方法流式细胞术检测50例NAFL患者以及50例健康对照者外周血CD4＋CD25＋Treg的变化，采用成组t检验或Mann-WhitneyU检验及Spearman秩相关检验进行统计学分析。结果NAFL患者外周血CD4＋CD25＋Treg占CD4＋T淋巴细胞的比例为（5．39±1．94）％，高于健康对照者的（4．21±1．52）％，差异有统计学意义（t=3．385，P〈O．01）。Treg数量与三酰甘油（TG）、体质指数（BMI）水平呈正相关（r=0．307、0．251，P=0．002、0．012），与高密度脂蛋白胆固醇（HDL—C）呈负相关（r=-0．306，P=0．002）。Treg在高BMI组、高TG组、低HDL，C组、高血压组及代谢综合征（MS）组升高（t=2．294、2．533、3．154、2．010、4．454，均P〈0．05），在高空腹血糖组差异无统计学意义（U=1143．500，P=0．471）。结论NAFL患者外周血Treg数量增加，可能是NAFL作为MS组分之一，与MS时机体处于慢性低度炎性反应状态所表现出的促炎与抗炎平衡紊乱有关。Objective To investigate the changes and clinical significance of the frequency of circulating CD4＋ CD25＋ regulatory T cells （Treg） in nonalcoholic fatty liver （NAFL） patients. Methods CD4＋ CD25＋ Treg in the peripheral blood from 50 NAFL patients and 50 healthy subjects were quantitatively analyzed using flow cytometry. Group t test or Mann-Whitney U test and Spearman＇s rank correlation test were used for statistical analysis. Results The proportion of circulating CD4＋CD25＋ Treg in NAFL patients was （5. 39 ± 1.94）%, which was significantly higher than that in healthy controls E（4.21± 1.52）%] （t= 3. 385, P〈0.01）. Further analysis revealed that the frequency of Treg was positively correlated with triglyceride （TG） level and body mass index （r= 0. 307 and 0. 251, respectively; P= O. 002 and 0. 012, respectively）, and negatively correlated with high density lipoprotein- cholesterol （HDL-C） （r=-0. 306, P=0. 002）. Meanwhile, Treg in patients with high body mass index, high TG, low HDL-C, hypertension and metabolic syndrome （MS） were all higher than those in controls （t=2. 294, 2. 533, 3. 154, 2. 010 and 4. 454, respectively; all P〈0.05）. But there was no significant difference between patients with high fasting blood glucose and controls （U = 1143. 500, P = 0. 471）. Conclusion The increased frequency of peripheral Treg in NAFL patients may have some relations with the imbalance of proinflammation and anti-inflammation in NAFL patients who coexisting with MS.

关 键 词：脂肪肝 代谢综合征 CD4阳性T淋巴细胞 

分 类 号：R575.5[医药卫生—消化系统]