肝细胞癌甲胎蛋白表达与肝祖细胞激活状态及患者预后的关系  

Association of alpha fetoprotein in hepatocellular carcinoma with activation of hepatic progenitor cells and patient prognosis

作　　者：钱光阳[1,2] 徐敏晖[2] 李建军[3] 蔡雄[2] 张长松[2] 吴孟超[2] 吴东[2] 卫立辛[2] 

机构地区：[1]苏州大学研究生院,苏州215006 [2]第二军医大学东方肝胆外科医院肿瘤免疫实验室,上海200438 [3]中国人民解放军总政治部机关门诊部,北京100120

出　　处：《第二军医大学学报》2012年第2期136-139,共4页Academic Journal of Second Military Medical University

基　　金：国家自然科学基金(30870974;30801347;30901722;31171321;81000970;81101622)~~

摘　　要：目的探讨原发性肝细胞癌组织中甲胎蛋白(AFP)表达与肝祖细胞激活状态及患者预后的关系。方法回顾性分析92例接受根治性切除术的原发性肝癌患者资料。其中男性76例,女性16例;年龄25～72岁,中位年龄51岁。对手术病理标本进行AFP和细胞角蛋白19(CK19)染色观察并定量。采用单因素Kaplan-Meier生存分析,观察AFP的表达对术后生存时间的影响。Cochran-Armitage线性趋势检验分析祖细胞激活状态(CK19阳性染色)评分与AFP表达的相关性。结果肝癌组织AFP高表达组总生存期短于肝癌组织AFP低表达组(P=0.021)。祖细胞激活状态评分与血清AFP浓度(P=0.046)、肝癌组织AFP表达(P<0.001)正相关。结论肝细胞癌甲胎蛋白高表达对肝癌预后不利,可能与肝脏祖细胞的激活状态有关。Objective To investigate the relationship of alpha fetoprotein（AFP） with activation of hepatic progenitor cells in hepatocellular carcinoma（HCC） and the prognosis of HCC patients.Methods The clinical data of 92 HCC patients who received curative hepatectomy were retrospectively analyzed.The patients included 76 males and 16 females,with a median age of 51（ranging 25-72） years.Expression of AFP and CK19 protein was immunohistochemically stained and quantified.Kaplan-Meier survival analysis was used to observe the influence of AFP expression on postoperative survival of patients.Cochran-armitage trend test was used to analyse the association of AFP expression with activation of progenitor cells（CK19 staining）.Results Patients with high AFP expression had a significantly shorter survival than those with low AFP expression（P=0.021）.The activation score of hepatic progenitor cells was positively correlated with serum AFP concentration（P=0.046） and AFP expression in tumor tissues（P0.001）.Conclusion AFP expression in HCC tissue predicts a poor prognosis.AFP is closely associated with activation of hepatic progenitor cells.

关 键 词：肝肿瘤 肝细胞癌 甲胎蛋白类 祖细胞 预后 

分 类 号：R735.7[医药卫生—肿瘤]