前列腺癌细胞系中STAT3活性与顺铂敏感性之间的关系  

Relationship of STAT3 activity with chemosensitivity to cisplatin in prostate cancer cell lines

作　　者：韩慧[1] 李春燕[1] 刘希[1] 储黎[1] 许青[1] 

机构地区：[1]同济大学附属第十人民医院肿瘤及放射治疗科,上海200072

出　　处：《第二军医大学学报》2012年第2期150-154,共5页Academic Journal of Second Military Medical University

基　　金：国家自然科学基金(30872591)~~

摘　　要：目的研究前列腺癌细胞系中STAT3活性与顺铂敏感性之间的关系。方法通过免疫细胞化学和蛋白质印迹法(Western blotting)检测3种常见前列腺癌细胞系LNCaP、PC3、DU145基础STAT3的活性;选取激素非依赖性细胞系PC3、DU145,分别加入2 ng/ml、20 ng/ml、200 ng/ml、2μg/ml、20μg/ml顺铂检测细胞增殖抑制情况,蛋白质印迹法检测低浓度顺铂作用DU145后STAT3的活性变化。结果激素非依赖性前列腺癌细胞系PC3、DU145中STAT3的活性较激素依赖性细胞系LNCaP中的高,且STAT3活性较低的PC3细胞较DU145细胞对顺铂更敏感,低浓度顺铂长时间作用DU145后可引起STAT3活性上调。结论 STAT3可能参与调节前列腺癌细胞对顺铂的敏感性。Objective To investigate the relationship between STAT3 activity and sensitivity to cisplatin in prostate cancer cell lines.Methods STAT3 activity was examined by immunocytochemistry and Western blotting analysis in three prostate cancer cell lines： LNCaP,PC3 and DU145.Androgen-independent cell lines PC3 and DU145 were selected to examine the inhibitory effect of various concentrations of cisplatin（2 ng/ml,20 ng/ml,200 ng/ml,2 μg/ml and 20 μg/ml）.STAT3 activity of DU145 cell line was re-examined by Western blotting analysis after treatment with low concentration of cisplatin.Results The STAT3 activity of androgen-independent cell lines PC3 and DU145 was higher than that of androgen-dependent cell line LNCaP.The sensitivity of DU145 cells to cisplatin was lower than that of PC3 cells with lower STAT3 activity.Treatment with low concentration of cisplatin for a long period caused STAT3 activation in DU145.Conclusion Our results suggest that STAT3 may play a role in regulating the sensitivity of prostate cancer cells to cisplatin.

关 键 词：STAT3转录因子 顺铂 前列腺肿瘤 

分 类 号：R737.25[医药卫生—肿瘤]