Notch-1信号通路与甲状腺乳头状癌的关系  

The Relationship of Notch-1 Signaling Pathway with Human Papillary Carcinoma of Thyroid

作　　者：杨晶金[1] 姚军[2] 沈峰清[1] 陈佳玉[2] 杨林军[3] 张强[3] 金晓燕[3] 梁勇[2,3] 

机构地区：[1]温州医学院第一临床学院,温州325000 [2]台州学院医学院,台州318000 [3]台州学院医学院附属台州市立医院肿瘤科,台州318000

出　　处：《中国细胞生物学学报》2012年第2期120-126,共7页Chinese Journal of Cell Biology

基　　金：国家自然科学基金(No.81072209)资助项目~~

摘　　要：研究Notch-1信号通路中Notch-1、NICD、Hes1、c-Myc与人甲状腺乳头状癌的关系,探讨Notch-1信号通路在甲状腺乳头状癌中的分子机制。对照人甲状腺乳头状癌及正常甲状腺组织标本各35例,采用Real Time PCR检测Notch-1、Hes1、c-Myc的mRNA表达情况,采用免疫组织化学、Western blot方法检测组织标本中Notch-1、NICD、Hes1、c-Myc蛋白的表达情况。Notch-1、Hes1在甲状腺乳头状癌中的mRNA表达水平明显降低,c-Myc mRNA的表达在甲状腺乳头状癌中升高,Notch-1、NICD、Hes1蛋白在人甲状腺乳头状癌中的表达低于正常甲状腺组织(P<0.05),c-Myc蛋白在甲状腺乳头状癌中的表达高于正常甲状腺组织(P<0.05)。甲状腺乳头状癌组织标本中,Notch-1信号通路中Notch-1、NICD、Hes1在基因转录和蛋白质表达水平上均明显下调,而c-Myc的基因转录和蛋白质表达水平均升高,提示Notch-1信号通路在甲状腺乳头状癌的发生发展中起抑癌作用,c-Myc基因表达升高与Notch-1信号通路可能无关。To approach the role of Notch-1 signaling pathway in the pathological development of human papillary carcinoma of thyroid,the expression levels of Notch-1,Hes1 and c-Myc mRNA were determined by Real Time PCR in 35 specimen of human papillary carcinoma of thyroid and 35 specimen of normal thyroid tissues around the nodular goiter.The Notch-1,NICD,Hes1 and c-Myc protein levels were determined by immunohistochemistry and Western blot.The expression of Notch-1 and Hes1 was significantly lower in the papillary carcinoma of thyroid than that in the normal thyroid tissues,but the expression of c-Myc was higher in the papillary carcinoma of thyroid.It suggested that Notch-1 and Hes1 may play a role in the pathological development of human papillary carcinoma of thyroid,and the expression of oncogene c-Myc may be unconcerned with the Notch-1 signaling pathway.

关 键 词：甲状腺乳头状癌 Notch-1信号通路 NOTCH-1 NICD HES1 C-MYC 

分 类 号：R736.1[医药卫生—肿瘤]