GP73在肝细胞癌诊断中的价值  

The diagnostic value of serum Golgi protein-73 in hepatocellular carcinoma

作　　者：梁嵘[1] 罗小玲[1] 葛莲英[1] 袁卫平[1] 岳惠芬[1] 贾保昌[1] 沈筱芸[1] 

机构地区：[1]广西医科大学附属肿瘤医院实验研究部,南宁530021

出　　处：《肿瘤》2012年第2期115-118,共4页Tumor

基　　金：广西科学基金资助项目(编号:桂科自0832235);广西科学研究与技术开发计划资助项目(编号:桂科攻0816004-13);广西科学研究与技术开发计划资助项目(编号:桂科攻1140003A-32);2011年广西研究生教育创新计划资助项目(编号:2011105981002D28)

摘　　要：目的:探讨GP73(Golgi protein-73)在肝细胞癌诊断中的价值。方法:收集外周血血清504例,其中肝细胞癌144例、肝硬化50例、乙型病毒性肝炎100例、乙型肝炎病毒携带者84例、其他恶性肿瘤50例、肝良性肿瘤26例和健康志愿者50例;应用双抗体夹心酶联免疫定量测定方法和电化学发光法检测血清中GP73和甲胎蛋白(α-fetoprotein,AFP)的表达水平。结果:肝细胞癌组血清GP73的表达水平显著高于其他各组(P<0.05);受试者工作特征(receiver operating characteristic,ROC)曲线设定GP73临界值为64ng/mL时,GP73诊断肝细胞癌的灵敏度和特异度分别为83.3%和88.3%,显著高于AFP(72.2%和76.7%),差异有统计学意义(P<0.05);血清GP73联合AFP检测诊断肝细胞癌的灵敏度可达94.4%。结论:血清GP73的表达水平用于诊断肝细胞癌的灵敏度和特异度优于AFP,血清GP73联合AFP检测可提高肝细胞癌的诊断率。Objective：To investigate the diagnostic value of serum Golgi protein-73（GP73） in hepatocellular carcinoma（HCC）.Methods：The peripheral serum samples from 504 individuals were collected,including 144 HCC patients,50 cirrhotic patients,100 hepatitis B patients,84 hepatitis B virus carriers,50 patients with other malignancies,26 patients with benign liver tumor,and 50 healthy volunteers.The expression levels of serum GP73 and alpha-fetal protein（AFP） were detected by double antibody sandwich ELISA and electrochemiluminescence assay,respectvely.Results：The expression level of serum GP73 from the HCC patients was significantly higher than those from the other patient groups（P0.05）.When the cut-off value was determined as 64 ng/mL by the receiver operating characteristic（ROC） curve,the sensitivity and specificity of GP73 for the diagnosis of HCC were both higher than those of AFP（83.3% vs 72.2%;88.3% vs 76.7%;both P0.05）.The sensitivity of GP73 in combination with serum AFP for the diagnosis of HCC was 94.4%.Conclusion：The sensitivity and specificity of serum GP73 for the diagnosis of HCC are higher than those of serum AFP.The rate of HCC diagnosis is increased by combined examination of serum GP73 and AFP.

关 键 词：癌 肝细胞 血清 诊断 GOLGI protein-73 甲胎蛋白 

分 类 号：R735.7[医药卫生—肿瘤]