胰岛素对人胰腺癌PANC1细胞增殖与侵袭能力的影响  

Effects of insulin on proliferation and invasion of human pancreatic cancer PANC1 cells

作　　者：刘涛[1] 王统玲[1] 勾善淼[1] 殷涛[1] 汪理[1] 周伟[1] 李永峰[1] 王春友[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院胰腺外科,湖北武汉430022

出　　处：《中华胰腺病杂志》2012年第1期19-21,共3页Chinese Journal of Pancreatology

基　　金：国家自然科学基金（30801098）

摘　　要：目的研究胰岛素对人胰腺癌PANC1细胞增殖、侵袭能力及细胞低氧诱导因子（HIF．1d）、血管内皮生长因子（VEGF）表达的影响。方法采用0．1、1、10、100nmol／L胰岛素处理PANC1。噻唑蓝法和Transwell小室侵袭实验检测细胞增殖和侵袭能力；蛋白质印迹法和实时PCR法检测增殖性细胞核抗原（PCNA）及HIF—1α、VEGF蛋白和mRNA的表达。结果胰岛素呈剂量依赖性诱导PANC1细胞的增殖，上调HIF-1α、VEGF蛋白表达。100nmol／L胰岛素干预4d，PANC1细胞的PCNA蛋白表达显著上调（1．196±0．014比1．157±0．013，P〈0．05）；Transwell小室穿膜细胞数量显著增加[（141．0±2．1）个比（89．0±1．4）个，P〈0．05]；HIF—1α蛋白表达显著上调（1．139±0．020比0．598-t-O．013，P〈0．05），但HIF-1α mRNA表达无明显变化；VEGF蛋白表达显著上调（1．011±O．023比0．6274-0．013，P〉0．05），VEGFmRNA表达亦显著上调（0．970±0．016比0．350±0．013，P〈0．05）。结论高胰岛素微环境可诱导PANC1细胞增殖，并通过上调HIF-1α及VEGF的表达增强细胞的侵袭能力。Objective To investigate the effects of insulin on the proliferation and invasion of human pancreatic cancer cells PANC1, and on its HIF-1α, VEGF expression. Methods PANC1 was pretreated with insulin of different concentrations （0.1, 1, 10, 100 nmol/L）. The proliferation of PANC1 was tested by MTT method, and transwell assay was used to test the invasion ability of PANC1. HIF-1α, VEGF and PCNA protein expression was assessed by Western blots, and HIF-1α, VEGF mRNA was detected by real-time PCR. Results Insulin could increase the proliferation of PANC1 in a dose-dependent manner （p 〈 0.05 ）, and increase the expression of HIF-1α, VEGF protein. After 100 nmol/L insulin treatment for 4 d, the PCNA protein expression in the insulin group was significantly higher than that in the control group （ 1. 196± 0. 014 vs 1. 157 ± 0. 013, P 〈 0.05）. The cancer cells passed through the chamber in insulin group were much more than that in the control group （ 141.0 ±2.1 vs 89.0 ± 1.4, P 〈0.05 ）. The expression of HIF-1α protein was significantly increased （ 1. 139 ± 0. 020 vs 0. 598 ±0. 013, P 〈0.05 ）, while there was no significant change of HIF-1α mRNA expression. Both the expression of VEGF protein and mRNA were significantly increased （ 1.011 ±0. 023 vs O. 627 ± 0. 013 0. 970 ± 0. 016 vs 0. 350 ＋ 0. 013, P 〈 0. 05 ）. Conclusions High insulin microenvironment could enhance the proliferation and invasion of PANC1 cells by up-regulating the expression of HIF-1α and VEGF.

关 键 词：胰腺肿瘤 胰岛素 增殖 侵袭 微环境 

分 类 号：R735.9[医药卫生—肿瘤]